Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu 210009, PR China.
Department of Environmental Occupational Health, Taizhou Center for Disease Control and Prevention, No.318 Yongtai Road, Hailing District, Taizhou City, Jiangsu Province, PR China.
Pathol Res Pract. 2020 Sep;216(9):153049. doi: 10.1016/j.prp.2020.153049. Epub 2020 Jun 6.
Non-small cell lung cancer (NSCLC) is a major type of lung cancer with high morbidity and mortality. Long non-coding RNAs (lncRNAs) have been reported to be important in development and progression of NSCLC. However, the role of lncRNA SFTA1P remains unclear. This study aims to explore the clinical roles, biological function, and mechanism of SFTA1P in NSCLC. SFTA1P expression was estimated by the quantitative real-time polymerase chain reaction (qRT-PCR) of 90 pairs of tissue samples, the Cancer Genome Atlas (TCGA) database and microarray. After overexpressing SFTA1P, NSCLC cell proliferation, cycle, and apoptosis were detected. We found that the expression of SFTA1P was significantly downregulated in NSCLC tissues with high diagnostic value (AUC = 0.87), which was consistent with the results of TCGA and microarray data. For the analysis of clinical features, the results revealed that SFTA1P expression was closely related to the pathological type (P < 0.01). Furthermore, the cell function results suggested that the overexpression of SFTA1P triggered cell cycle arrest in the S-phase (P < 0.05). From a mechanistic perspective, the results showed that the PI3K-AKT signaling pathway was inhibited after overexpression of SFTA1P in NSCLC. Taken together, this work supported that SFTA1P may play a suppressing role in the tumorigenesis of NSCLC by modulating PI3K-AKT signaling pathway to influence cell cycle, which provides a potential and prospective biomarker for NSCLC.
非小细胞肺癌（NSCLC）是一种发病率和死亡率都很高的主要肺癌类型。长链非编码 RNA（lncRNA）已被报道在 NSCLC 的发生和发展中具有重要作用。然而，lncRNA SFTA1P 的作用尚不清楚。本研究旨在探讨 SFTA1P 在 NSCLC 中的临床作用、生物学功能和机制。通过 90 对组织样本、癌症基因组图谱（TCGA）数据库和微阵列的实时定量聚合酶链反应（qRT-PCR）来估计 SFTA1P 的表达。过表达 SFTA1P 后，检测 NSCLC 细胞的增殖、周期和凋亡。我们发现 SFTA1P 的表达在 NSCLC 组织中明显下调，具有较高的诊断价值（AUC=0.87），与 TCGA 和微阵列数据的结果一致。对临床特征的分析结果表明，SFTA1P 的表达与病理类型密切相关（P<0.01）。此外，细胞功能结果表明，SFTA1P 的过表达导致细胞周期在 S 期停滞（P<0.05）。从机制角度来看，结果表明，SFTA1P 在 NSCLC 中过表达后，PI3K-AKT 信号通路被抑制。综上所述，这项工作表明，SFTA1P 可能通过调节 PI3K-AKT 信号通路影响细胞周期，在 NSCLC 的肿瘤发生中发挥抑制作用，为 NSCLC 提供了一个有潜力和有前景的生物标志物。
