Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China; The Key Laboratory of Biodiversity Conservation in Karst Mountain Area of Southwest of China, Forestry Ministry, School of Life Sciences, Guizhou Normal University, Guiyang, 550003, China; Key Laboratory of Plant Physiology and Developmental Regulation, School of Life Sciences, Guizhou Normal University, Guiyang, 550003, China.
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Plant Physiol Biochem. 2020 Oct;155:472-482. doi: 10.1016/j.plaphy.2020.08.019. Epub 2020 Aug 8.
Exploration of native microbes is a feasible way to develop microbial agents for ecological restoration. This study was aimed to explore the impact of Bacillus amyloliquefaciens PDR1 from karst adaptive plant on the activity of root plasma membrane H-ATPase in Arabidopsis thaliana. A. thaliana was cultured in presence or absence of B. amyloliquefaciens PDR1 and its effects on the growth were evaluated by measuring the taproot length and dry weight. The rhizosphere acidification capacity was detected by a pH indicator, a pH meter and non-invasive micro-test techniques (NMT). The nutrient uptake was performed using appropriate methods. A combination of transcriptome sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to measure the expression of functional genes that regulate the plasma membrane H-ATPase activity in A. thaliana roots. Functional analysis was performed to understand how B. amyloliquefaciens regulates biological processes and metabolic pathways to strengthen A. thaliana resistance to alkaline stress. Here, we show that volatile organic compounds (VOCs) from B. amyloliquefaciens PDR1 promoted the growth and development of A. thaliana, enhanced the plasma membrane H-ATPase activity, and affected ion absorption in Arabidopsis roots. Moreover, B. amyloliquefaciens PDR1 VOCs did not affect the expression of the gene coding for plasma membrane H-ATPase, but affected the expression of genes regulating the activity of plasma membrane H-ATPase. Our findings illuminate the mechanism by which B. amyloliquefaciens regulates the growth and alkaline stress resistance of A. thaliana, and lay a foundation for wide and efficient application for agricultural production and ecological protection.
探索土著微生物是开发用于生态修复的微生物制剂的可行方法。本研究旨在探讨来自喀斯特适应植物的解淀粉芽孢杆菌 PDR1 对拟南芥根质膜 H+-ATP 酶活性的影响。在存在或不存在解淀粉芽孢杆菌 PDR1 的情况下培养拟南芥，并通过测量主根长度和干重来评估其对生长的影响。通过 pH 指示剂、pH 计和非侵入性微测技术（NMT）检测根际酸化能力。使用适当的方法进行养分吸收。结合转录组测序和实时定量聚合酶链反应（qRT-PCR）测量调节拟南芥根质膜 H+-ATP 酶活性的功能基因的表达。进行功能分析以了解解淀粉芽孢杆菌如何调节生物过程和代谢途径以增强拟南芥对碱性胁迫的抗性。在这里，我们表明解淀粉芽孢杆菌 PDR1 的挥发性有机化合物（VOCs）促进了拟南芥的生长和发育，增强了质膜 H+-ATP 酶活性，并影响了拟南芥根中的离子吸收。此外，解淀粉芽孢杆菌 PDR1 VOCs 不影响编码质膜 H+-ATP 酶的基因的表达，但影响调节质膜 H+-ATP 酶活性的基因的表达。我们的研究结果阐明了解淀粉芽孢杆菌调节拟南芥生长和耐碱性胁迫的机制，为农业生产和生态保护的广泛高效应用奠定了基础。
