Research Institutes of Sweden (RISE), Norrköping, Sweden.
Dipartimento di Chimica - Università degli Studi di Bari "A. Moro", Bari, Italy; CSGI (Centre for Colloid and Surface Science), Unit of Bari, Bari, Italy; Center for Functional materials, The Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland.
Methods Enzymol. 2020;642:403-433. doi: 10.1016/bs.mie.2020.07.003. Epub 2020 Jul 24.
There is an increasing interest for low cost, ultrasensitive, time saving yet reliable, point-of-care bioelectronic sensors. Electrolyte gated organic field effect transistors (EGOFETs) are proven compelling transducers for various sensing applications, offering direct electronic, label-free transduction of bio-recognition events along with miniaturization, fast data handling and processing. Given that field effect transistors act as intrinsically signal amplifiers, even a small change of a chemical or biological quantity may significantly alter the output electronic signal. In EGOFETs selectivity can be guaranteed by the immobilization of bioreceptors able to bind specifically a target analyte. The layer of receptors can be linked to one of the electronic active interfaces of the transistor, and the interactions with a target molecule affect the electronic properties of the device. The present chapter discusses main aspects of EGOFETs transducers along with detailed examples of how to tailor the device interfaces with desired functionality. The development of an "electronic tongue" based on an EGOFET device coupled to odorant binding proteins (OBPs) for enantiomers differentiation is presented.
人们对于低成本、超灵敏、节省时间但可靠的即时生物电子传感器越来越感兴趣。电解质门控有机场效应晶体管（EGOFET）已被证明是各种传感应用的极具吸引力的换能器，可直接进行生物识别事件的电子、无标记转换，同时实现小型化、快速数据处理。由于场效应晶体管充当固有信号放大器，即使是化学或生物数量的微小变化也可能显著改变输出电子信号。在 EGOFET 中，可以通过固定能够特异性结合目标分析物的生物受体来保证选择性。受体层可以与晶体管的一个电子活性界面相连，与目标分子的相互作用会影响器件的电子特性。本章讨论了 EGOFET 换能器的主要方面，并详细介绍了如何根据需要的功能来定制器件接口。介绍了一种基于与气味结合蛋白（OBP）偶联的 EGOFET 器件的“电子舌”，用于对映体的区分。
