Szabó G, Bunce C, Brown G, Klein G, Sümegi J
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Exp Cell Res. 1988 Apr;175(2):334-43. doi: 10.1016/0014-4827(88)90197-8.
We have investigated the possible relationship between c-myc gene activity and other variable traits in HL60. In a panel of variant lines, a good correlation was observed between myc gene copy number and the level of myc mRNA. There was no correlation between myc amplification or expression and the resistance of the lines to induction of terminal neutrophilic or monocytic differentiation. Therefore, myc mRNA level does not appear to determine the ability of the variant HL60 lines to respond to inducers of differentiation. Flow cytometric analyses of the expression of a differentiation antigen (AGF 4.36) revealed stable negative and positive subpopulations in growing HL60. myc amplification, expression, and inducibility were identical in these subpopulations, suggesting that variation of these traits in HL60 sublines and variants is not due to maturation state differences. myc gene copy number was also identical in transferrin receptor positive (proliferating) and negative (resting) populations. These data contradict the notion that myc amplification has been important in determining the in vitro biological properties of HL60.
我们研究了HL60中c-myc基因活性与其他可变性状之间的可能关系。在一组变异株系中，观察到myc基因拷贝数与myc mRNA水平之间存在良好的相关性。myc扩增或表达与株系对诱导终末嗜中性或单核细胞分化的抗性之间没有相关性。因此，myc mRNA水平似乎并不能决定变异HL60株系对分化诱导剂的反应能力。对一种分化抗原（AGF 4.36）表达的流式细胞术分析显示，生长中的HL60存在稳定的阴性和阳性亚群。这些亚群中的myc扩增、表达和诱导性是相同的，这表明HL60亚系和变异株中这些性状的变化并非由于成熟状态差异。转铁蛋白受体阳性（增殖）和阴性（静止）群体中的myc基因拷贝数也相同。这些数据与myc扩增在决定HL60体外生物学特性方面很重要这一观点相矛盾。
