Graham S V, Tindle R W, Birnie G D
Leuk Res. 1985;9(2):239-47. doi: 10.1016/0145-2126(85)90086-4.
Three sublines of the human promyelocytic leukaemia cell line HL60 have been isolated. Histochemical, karyotypic and immunological analyses have confirmed the identity of the three sublines as HL60. However, the extent to which c-myc homologous DNA sequences are amplified in the genomes of these cells varies from 4- to 30-fold. Moreover, the relative abundance of c-myc-related RNA sequences in uninduced cells of each subline is directly proportional to the gene copy number. It is concluded that, while high levels of the c-myc gene and its transcripts may have had a role in the establishment of HL60 cells, these are not required for maintenance of the HL60 phenotype. However we cannot rule out the possibility that a low level of amplification and enhancement of transcription has a role in maintenance of this phenotype.
已分离出人类早幼粒细胞白血病细胞系HL60的三个亚系。组织化学、核型分析和免疫学分析已证实这三个亚系与HL60一致。然而，这些细胞基因组中c-myc同源DNA序列的扩增程度在4至30倍之间变化。此外，每个亚系未诱导细胞中c-myc相关RNA序列的相对丰度与基因拷贝数成正比。得出的结论是，虽然高水平的c-myc基因及其转录本可能在HL60细胞的建立中起作用，但维持HL60表型并不需要它们。然而，我们不能排除低水平的扩增和转录增强在维持该表型中起作用的可能性。
