Department of Chemistry, University of Agriculture, ul. Balicka 122, 30-149 Kraków, Poland.
Department of Animal Product Technology, Faculty of Food Technology, University of Agriculture, ul. Balicka 122, 30-149 Kraków, Poland.
Food Chem. 2021 Feb 15;338:127867. doi: 10.1016/j.foodchem.2020.127867. Epub 2020 Aug 17.
This is the first time that active double-layered furcellaran/gelatin hydrolysate films containing Ala-Tyr peptide were developed and characterised for their properties. Afterwards, films were used on Atlantic mackerel stored at -18 °C for 4 months and samples were analysed for changes in their microbiological quality, TVB-N, biogenic amine content, fatty acid composition and TBARS. Active films had higher TS (13.4 MPa) and lower WS (62.8%). The films showed no DPPH radical scavenging properties but high FRAP (6.6 mMol Trolox/mg). No significant effects on the oxidation of fish samples were observed with TBARS increasing from 12.04 to 22.50 mg/kg. Freezing successfully inhibited the growth of microorganisms and no differences in microbiological growth or biogenic amine formation were observed. However, the application of films inhibited the formation of TVB-N. Antimicrobiological properties of the film should be further investigated during storage of perishable food products at temperatures above 0 °C.
这是首次开发并对含有 Ala-Tyr 肽的活性双层 furcellaran/明胶水解物薄膜的特性进行研究。之后，将薄膜用于在-18°C下储存的大西洋马鲛鱼，储存 4 个月后对其微生物质量、TVB-N、生物胺含量、脂肪酸组成和 TBARS 的变化进行分析。活性薄膜的拉伸强度（TS）更高（13.4 MPa），水分迁移率（WS）更低（62.8%）。薄膜没有 DPPH 自由基清除特性，但具有较高的 FRAP（6.6 mMol Trolox/mg）。TBARS 从 12.04 增加到 22.50 mg/kg，对鱼样的氧化没有明显影响。冷冻成功抑制了微生物的生长，微生物生长或生物胺形成没有差异。然而，薄膜的应用抑制了 TVB-N 的形成。在 0°C 以上温度储存易腐食品时，应进一步研究薄膜的抗菌性能。
