Schiaffino S, Gorza L, Pitton G, Saggin L, Ausoni S, Sartore S, Lømo T
Institute of General Pathology, University of Padova, Italy.
Dev Biol. 1988 May;127(1):1-11. doi: 10.1016/0012-1606(88)90183-2.
Using immunofluorescence procedures with specific polyclonal and monoclonal antimyosin antibodies we have found that embryonic and neonatal myosin heavy chains (MHCs), which in rat skeletal muscle disappear during the first weeks after birth, are reexpressed in adult muscle after denervation. Reactivity for embryonic and neonatal MHCs was detected in some fibers as early as 3 days after denervation, became more evident by 7 days, and occurred exclusively in the type 2A fiber population. Paralysis of innervated muscles by tetrodotoxin block of the sciatic nerve also resulted in the reappearance of embryonic and neonatal MHCs in type 2A fibers. Significant variation in the degree of immunoreactivity was observed in different segments of the same muscle fiber, suggesting that coordination of muscle fiber nuclei in the control of myosin heavy chain gene expression is partially lost following denervation.
使用针对特定多克隆和单克隆抗肌球蛋白抗体的免疫荧光程序，我们发现，大鼠骨骼肌中的胚胎和新生肌球蛋白重链（MHCs）在出生后的头几周消失，但在去神经支配后的成年肌肉中会重新表达。去神经支配后3天，在一些纤维中就检测到了胚胎和新生MHCs的反应性，到7天时变得更加明显，且仅出现在2A型纤维群体中。通过坐骨神经的河豚毒素阻断使受神经支配的肌肉麻痹，也导致2A型纤维中出现胚胎和新生MHCs。在同一肌纤维的不同节段观察到免疫反应程度的显著差异，这表明去神经支配后，肌纤维核在肌球蛋白重链基因表达控制中的协调性部分丧失。
