Department of Obstetrics and Gynecology, Keio University School of Medicine, Japan; Department of Obstetrics and Gynecology, National Hospital Organization Tokyo Medical Center, Japan.
Department of Obstetrics and Gynecology, Keio University School of Medicine, Japan; Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Japan.
Gynecol Oncol. 2020 Nov;159(2):329-334. doi: 10.1016/j.ygyno.2020.07.106. Epub 2020 Aug 20.
To (i) identify correlations between selected immunogenic factors and clinicopathological characteristics, (ii) determine whether intratumoral abundance of various specific tumor-infiltrating lymphocytes (TILs) is a prognostic indicator in women with Stage II and III cervical cancer who undergo treatment with cisplatin-based concurrent chemoradiotherapy (CCRT), and (iii) investigate subtypes of FOXP3 T cells in 15 fresh samples of cervical cancer.
In this retrospective study, intratumoral lesions in colposcopic biopsies from 55 women with advanced cervical cancer who subsequently underwent CCRT at our institution were subjected to automatic immunological staining using the following six mouse monoclonal antibodies: anti-CD3, anti-CD4, anti-CD8, anti-CD20, anti-CD206, and anti-FOXP3. Associations between the findings on automatic scoring of the number of each type of TIL in each specimen and various clinicopathological characteristics were analyzed, as were associations between the abundance of various specific types of TIL and survival. Subtypes of FOXP3 TILs in 15 additional fresh tumor samples were also investigated using flow cytometry.
Infiltration with CD8 TILs was associated with pelvic lymph node metastasis. Abundant infiltration by CD3, CD4, CD8, CD206, and FOXP3 TILs were statistically significant indicators of better progression-free and overall survival. Regarding subtypes of FOXP3 TILs, non-Tregs (Fr-III) were found in all samples tested for this.
The abundance of various specific intratumoral TILs may be prognostic indicators in patients with advanced cervical cancer undergoing CCRT.
（i）确定选定的免疫原性因素与临床病理特征之间的相关性，（ii）确定在接受顺铂为基础的同期放化疗（CCRT）治疗的 II 期和 III 期宫颈癌女性中，肿瘤内各种特定肿瘤浸润淋巴细胞（TIL）的丰度是否是预后指标，（iii）研究 15 例宫颈癌新鲜样本中 FOXP3 T 细胞的亚型。
在这项回顾性研究中，对 55 名在我院接受 CCRT 的晚期宫颈癌女性的阴道镜活检肿瘤内病变进行了自动免疫染色，使用以下六种小鼠单克隆抗体：抗-CD3、抗-CD4、抗-CD8、抗-CD20、抗-CD206 和抗-FOXP3。分析了每个标本中每种 TIL 数量的自动评分结果与各种临床病理特征之间的关系，以及各种特定类型 TIL 的丰度与生存之间的关系。还使用流式细胞术研究了另外 15 个新鲜肿瘤样本中 FOXP3 TIL 的亚型。
CD8 TIL 的浸润与盆腔淋巴结转移有关。CD3、CD4、CD8、CD206 和 FOXP3 TIL 的丰富浸润是无进展生存和总生存的统计学显著指标。关于 FOXP3 TIL 的亚型，在所有测试的样本中都发现了非 Tregs（Fr-III）。
在接受 CCRT 的晚期宫颈癌患者中，各种特定肿瘤内 TIL 的丰度可能是预后指标。
