表皮生长因子（EGF）受体基因转录。对Sp1和一种EGF受体特异性因子的需求。
Epidermal growth factor (EGF) receptor gene transcription. Requirement for Sp1 and an EGF receptor-specific factor.
作者信息
Kageyama R, Merlino G T, Pastan I
机构信息
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
出版信息
J Biol Chem. 1988 May 5;263(13):6329-36.
We have studied in vitro transcription of the human epidermal growth factor (EGF) receptor proto-oncogene using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. With the in vitro system we found that Sp1 and other trans-acting factors bound to the EGF receptor promoter regions and are required for maximal expression. Fractionation showed that a DEAE-Sepharose fraction (BA) contained a novel factor, which specifically stimulated EGF receptor transcription 5- to 10-fold. The molecular mass of the native form of the factor is about 270-kDa based on its migration on Sephacryl S-300. This factor may activate transcription of the proto-oncogene through a weak or indirect interaction with the DNA template.
我们利用过度表达表皮生长因子（EGF）受体的A431人表皮样癌细胞的核提取物，对人表皮生长因子（EGF）受体原癌基因进行了体外转录研究。在体外系统中，我们发现Sp1和其他反式作用因子与EGF受体启动子区域结合，并且是最大表达所必需的。分级分离表明，二乙氨基乙基琼脂糖（DEAE-Sepharose）组分（BA）含有一种新因子，它能特异性地刺激EGF受体转录5至10倍。根据该因子在Sephacryl S-300上的迁移情况，其天然形式的分子量约为270 kDa。该因子可能通过与DNA模板的微弱或间接相互作用来激活原癌基因的转录。
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