Hao Miao, Wang Huiyu, Zhang Chu, Li Chunyan, Wang Xiaofeng
Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Oncol Lett. 2020 Oct;20(4):109. doi: 10.3892/ol.2020.11970. Epub 2020 Aug 10.
Oral squamous cell carcinoma is one of the most common causes of malignancy-associated death. Early diagnosis of oral squamous cell carcinoma (OSCC) is important in patient treatment and prognostic evaluation. Due to the lack of significant therapeutic benefit, the 5-year survival rate has not improved. Therefore, effective novel markers are needed to improve diagnosis. To determine novel promising diagnostic biomarkers for OSCC, 416 upregulated and 416 downregulated differentially expressed genes were screened from OSCC tissues using an RNA microarray. The results suggested that minichromosome maintenance protein () mRNA was significantly overexpressed in OSCC tissues compared with that in adjacent normal tissues. Moreover, silencing of MCM5 expression an OSCC cell line (SCC-15) significantly impaired proliferation and colony formation. Furthermore, negative regulation of the mRNA and protein expression of MCM5 and demonstrated that served as a cancer-promoting gene modulating OSCC cell proliferation through induced G/M phase arrest. In this process, the mRNA expression of cyclin E and cyclin-dependent kinase 2 was downregulated, while p21 expression was upregulated. These results suggested that MCM5 may be an important pathogenic factor of OSCC. High expression levels of MCM5 may serve as a marker for the early diagnosis of OSCC.
口腔鳞状细胞癌是恶性肿瘤相关死亡的最常见原因之一。口腔鳞状细胞癌（OSCC）的早期诊断对患者治疗和预后评估至关重要。由于缺乏显著的治疗益处，5年生存率并未提高。因此，需要有效的新型标志物来改善诊断。为了确定OSCC有前景的新型诊断生物标志物，使用RNA微阵列从OSCC组织中筛选出416个上调和416个下调的差异表达基因。结果表明，与相邻正常组织相比，微小染色体维持蛋白（）mRNA在OSCC组织中显著过表达。此外，在OSCC细胞系（SCC-15）中沉默MCM5表达显著损害细胞增殖和集落形成。此外，MCM5的mRNA和蛋白表达的负调控表明，通过诱导G/M期阻滞，作为促进癌症的基因调节OSCC细胞增殖。在此过程中，细胞周期蛋白E和细胞周期蛋白依赖性激酶2的mRNA表达下调，而p21表达上调。这些结果表明，MCM5可能是OSCC的重要致病因素。MCM5的高表达水平可能作为OSCC早期诊断的标志物。
