Srinivasan P, Selvankumar T, Paray Bilal Ahamad, Rehman Muneeb U, Kamala-Kannan S, Govarthanan M, Kim Woong, Selvam K
PG and Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal, Tamil Nadu 637501 India.
Department of Zoology, College of Science, King Saud University, Riyadh, 11451 Saudi Arabia.
3 Biotech. 2020 Aug;10(8):366. doi: 10.1007/s13205-020-02363-6. Epub 2020 Aug 1.
The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box-Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.
本研究探索了将漆酶固定在铁磁性纳米颗粒（MNPs）上，以增强其酶学性质和应用。使用Box-Behnken设计（BBD）对固定化过程进行了优化。BBD对带有实验数据的二次模型具有显著性。在漆酶浓度为0.75 mg/mL、MNPs为200 mg/mL、与碳二亚胺交联度为0.3%以及交联时间为3小时的条件下，观察到预测模型的漆酶活性回收率最高（99%）。使用振动样品磁强计（VSM）分析了MNPs的磁化活性（8 emu/g）以及与MNPs固定化的漆酶的磁化活性（4 emu/g）。在pH 7.0和55°C条件下观察到固定化漆酶的最大活性。固定化漆酶具有更高的稳定性（100小时）以及显著的毒死蜱（农药）降解活性。高效液相色谱（HPLC）结果证实了毒死蜱的降解代谢产物。总之，固定化漆酶优于游离漆酶，显示出良好的结构和应用特性。
