van Vreeswijk H, Ruiter D J, Bröcker E B, Welvaart K, Ferrone S
Department of Pathology, University Medical Center, Leiden, The Netherlands.
J Invest Dermatol. 1988 May;90(5):755-60. doi: 10.1111/1523-1747.ep12560951.
Fifty-five primary and 33 metastatic surgically removed melanoma lesions were stained in indirect immunoperoxidase with anti HLA-DR, DQ, and DP monoclonal antibodies and with the monoclonal antibody CL203.4 to a 96-K melanoma associated antigen (MAA). The latter antigen may represent a marker to monitor susceptibility of melanoma cells to modulation by IFN-gamma, because it is highly susceptible to induction by IFN-gamma. In primary melanomas 44%, 29%, 10%, and 55% of the lesions tested were evidently stained by anti HLA-DR, DQ, DP, and 96-K MAA monoclonal antibodies, respectively. A statistically significant association (P less than 0.01) was demonstrated between the degree of intratumoral lymphocytic infiltrate and the expression of HLA-DR and HLA-DQ antigens. In addition, a high degree of concordance in the reactivity pattern of individual lesions stained for HLA-DR antigens and for the 96-K MAA was found. In metastases 64%, 33%, 47%, and 100% of the lesions tested were evidently stained by anti HLA-DR, DQ, DP, and 96-K MAA monoclonal antibodies, respectively. This study indicates that HLA-DR and HLA-DP antigens are expressed in a higher percentage of metastatic than of primary melanomas and that there is no marked difference in the expression of HLA-DQ antigens between primary and metastatic melanomas. The data suggest that the regulatory mechanisms which control the expression of HLA-DR and DP antigens in primary and metastatic melanoma lesions are different. Locally produced IFN-gamma may play a role in the regulation of HLA Class II antigens in primary melanomas.
采用抗HLA-DR、DQ和DP单克隆抗体以及抗96-K黑色素瘤相关抗原（MAA）的单克隆抗体CL203.4，对55个手术切除的原发性黑色素瘤病灶和33个转移性黑色素瘤病灶进行间接免疫过氧化物酶染色。后一种抗原可能是监测黑色素瘤细胞对γ干扰素调节敏感性的标志物，因为它对γ干扰素的诱导高度敏感。在原发性黑色素瘤中，分别有44%、29%、10%和55%的检测病灶被抗HLA-DR、DQ、DP和96-K MAA单克隆抗体明显染色。肿瘤内淋巴细胞浸润程度与HLA-DR和HLA-DQ抗原表达之间存在统计学显著关联（P<0.01）。此外，发现针对HLA-DR抗原和96-K MAA染色的单个病灶的反应模式具有高度一致性。在转移灶中，分别有64%、33%、47%和100%的检测病灶被抗HLA-DR、DQ、DP和96-K MAA单克隆抗体明显染色。本研究表明，转移性黑色素瘤中HLA-DR和HLA-DP抗原的表达百分比高于原发性黑色素瘤，且原发性和转移性黑色素瘤之间HLA-DQ抗原的表达无明显差异。数据表明，控制原发性和转移性黑色素瘤病灶中HLA-DR和DP抗原表达的调节机制不同。局部产生的γ干扰素可能在原发性黑色素瘤中HLA II类抗原的调节中起作用。
