Museyi K, Van Dyck E, Vervoort T, Taylor D, Hoge C, Piot P
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
J Infect Dis. 1988 May;157(5):1039-43. doi: 10.1093/infdis/157.5.1039.
An experimental enzyme immunoassay (EIA) for detecting serum IgG antibody to Haemophilus ducreyi was developed using an ultrasonicated whole-cell antigen. The mean optical densities (OD) for sera from men with proven chancroid from Nairobi (47 patients) and Bangkok (72 patients) were significantly higher than those obtained from Nairobi men with genital ulcers not due to H. ducreyi, from Nairobi men with urethritis, from pregnant women in Nairobi, and from European men with sexually transmitted disease. When an OD of 0.500 was taken as the cutoff value, 89% and 55% of men with proven chancroid in Nairobi and Bangkok, respectively, were positive for H. ducreyi antibody, as compared with 2%-17% in the control groups. A rise in OD was observed in five of 18 patients with clinical chancroid. These results confirm the development of circulating antibodies in chancroid and suggest that this EIA may be useful for the diagnosis and epidemiological study of H. ducreyi infection.
利用超声破碎的全细胞抗原开发了一种用于检测血清中杜克雷嗜血杆菌IgG抗体的实验性酶免疫测定法（EIA）。来自内罗毕（47例患者）和曼谷（72例患者）确诊为软下疳的男性血清的平均光密度（OD）显著高于来自内罗毕非杜克雷嗜血杆菌所致生殖器溃疡的男性、内罗毕尿道炎男性、内罗毕孕妇以及患有性传播疾病的欧洲男性的血清平均光密度。当以0.500的OD值作为临界值时，内罗毕和曼谷确诊为软下疳的男性中，分别有89%和55%的人杜克雷嗜血杆菌抗体呈阳性，而对照组的阳性率为2%-17%。在18例临床诊断为软下疳的患者中，有5例观察到OD值升高。这些结果证实了软下疳患者体内循环抗体的产生，并表明这种EIA可能有助于杜克雷嗜血杆菌感染的诊断和流行病学研究。
