Roggen E L, Hoofd G, Van Dyck E, Piot P
Department of Infection and Immunity, Institute of Tropical Medicine, Antwerp, Belgium.
Sex Transm Dis. 1994 Jan-Feb;21(1):36-42. doi: 10.1097/00007435-199401000-00008.
Chancroid is a risk factor for heterosexually acquiring HIV. Controlling its spread may reduce HIV transmission.
To develop EIAs for assessing antibody levels and for seroepidemiologic studies.
Anti-Haemophilus ducreyi IgA, IgG and IgM EIAs were standardized using a crude cocktail antigen. Evaluation was on sera from Kenya, Rwanda, Thailand and The Gambia. The two-tailed student's t test was used to compare results.
The specificity of IgA was 97% (95% confidence interval (CI): 95-99%), of IgG was 92% (95% CI: 89-95%), and of IgM was 99% (95% CI: 98-100%). The sensitivity of IgA was 88% (95% CI: 83-93%), of IgG was 93% (95% CI:89-97%), and of IgM was 78% (95% CI:71-85%) in patients having an ulceration for more than eight days. Thus, 95% (95% CI:92-98%) of the chancroid patients were seropositive for at least one antibody type. The IgG and IgA EIAs were more sensitive in patients older than 24 years of age. Higher IgG rates were found in HIV infected chancroid patients.
The EIAs should be useful for studying the kinetics of antibody levels and the epidemiology of H. ducreyi infection.
软下疳是异性恋者感染HIV的一个危险因素。控制其传播可能会减少HIV的传播。
开发用于评估抗体水平和血清流行病学研究的酶免疫测定法。
使用粗制混合抗原对抗杜克雷嗜血杆菌IgA、IgG和IgM酶免疫测定法进行标准化。对来自肯尼亚、卢旺达、泰国和冈比亚的血清进行评估。采用双侧学生t检验比较结果。
IgA的特异性为97%（95%置信区间（CI）：95 - 99%），IgG为92%（95%CI：89 - 95%），IgM为99%（95%CI：98 - 100%）。在溃疡超过8天的患者中，IgA的敏感性为88%（95%CI：83 - 93%），IgG为93%（95%CI：89 - 97%），IgM为78%（95%CI：71 - 85%）。因此，95%（95%CI：92 - 98%）的软下疳患者至少有一种抗体类型呈血清阳性。IgG和IgA酶免疫测定法在24岁以上患者中更敏感。在感染HIV的软下疳患者中发现较高的IgG率。
这些酶免疫测定法应有助于研究抗体水平的动力学和杜克雷嗜血杆菌感染的流行病学。
