Zou Yunlong, Ma Dezun, Shen He, Zhao Yannan, Xu Bai, Fan Yongheng, Sun Zheng, Chen Bing, Xue Weiwei, Shi Ya, Xiao Zhifeng, Gu Rui, Dai Jianwu
China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun 130033, China.
Biomater Sci. 2020 Sep 21;8(18):5145-5156. doi: 10.1039/d0bm00431f. Epub 2020 Aug 24.
Neural stem/progenitor cell (NSPC)-based spinal cord injury (SCI) therapy is expected to bridge the lesion site by transplanting exogenous NSPCs for replacement of lost cells. The transplanted NSPCs produce a microenvironment conducive to neuronal regeneration, and ultimately, functional recovery. Although both human fetal brain- and spinal cord- derived NSPCs (hbNSPCs and hscNSPCs, respectively) have been used for SCI repair, it remains unclear whether hscNSPCs are a more appropriate stem cell source for transplantation than hbNSPCs. Therefore, in this study, we transplanted hbNSPCs or hscNSPCs into rats with complete transection SCI to monitor their differences in SCI treatment. An aligned collagen sponge scaffold (ACSS) was used here for cell retention. Aligned biomaterial scaffolds provide a support platform and favorable morphology for cell growth and differentiation, and guide axial axonal extension. The ACSS fabricated by our group has been previously reported to improve spinal cord repair by promoting neuronal regeneration and remyelination. Compared with the hbNSPC-ACSS, the hscNSPC-ACSS effectively promoted long-term cell survival and neuronal differentiation and improved the SCI microenvironment by reducing inflammation and glial scar formation. Furthermore, the transplanted hscNSPC-ACSS improved recovery of locomotor functions. Therefore, hscNSPCs appear to be a superior cell source to hbNSPCs for SCI cell therapy with greater potential clinical applications.
基于神经干细胞/祖细胞（NSPC）的脊髓损伤（SCI）治疗有望通过移植外源性NSPC来桥接损伤部位，以替代丢失的细胞。移植的NSPC产生有利于神经元再生的微环境，并最终实现功能恢复。尽管来自人胎儿脑和脊髓的NSPC（分别为hbNSPC和hscNSPC）都已用于SCI修复，但hscNSPC是否比hbNSPC更适合作为移植的干细胞来源仍不清楚。因此，在本研究中，我们将hbNSPC或hscNSPC移植到完全横断性SCI大鼠中，以监测它们在SCI治疗中的差异。此处使用排列的胶原海绵支架（ACSS）来保留细胞。排列的生物材料支架为细胞生长和分化提供了支持平台和有利的形态，并引导轴突轴向延伸。我们小组制造的ACSS此前已报道可通过促进神经元再生和髓鞘再生来改善脊髓修复。与hbNSPC-ACSS相比，hscNSPC-ACSS有效促进了长期细胞存活和神经元分化，并通过减少炎症和胶质瘢痕形成改善了SCI微环境。此外，移植的hscNSPC-ACSS改善了运动功能的恢复。因此，对于SCI细胞治疗而言，hscNSPC似乎是比hbNSPC更优越的细胞来源，具有更大的潜在临床应用价值。
