Tsoleridis Theocharis, Ball Jonathan K
Centre for Global Virus Infections, University of Nottingham, Nottingham, UK.
School of Life Sciences, University of Nottingham, Nottingham, UK.
Methods Mol Biol. 2020;2203:33-40. doi: 10.1007/978-1-0716-0900-2_2.
The recent emergence of SARS, SARS-CoV2 and MERS and the discovery of novel coronaviruses in animals and birds suggest that the Coronavirus family is far more diverse than previously thought. In the last decade, several new coronaviruses have been discovered in rodents around the globe, suggesting that they are the natural reservoirs of the virus. In this chapter we describe the process of screening rodent tissue for novel coronaviruses with PCR, a method that is easily adaptable for screening a range of animals.
近期SARS、SARS-CoV2和中东呼吸综合征(MERS)的出现,以及在动物和鸟类中发现新型冠状病毒,表明冠状病毒家族比之前认为的要多样化得多。在过去十年中,全球各地的啮齿动物身上发现了几种新型冠状病毒,这表明它们是该病毒的天然宿主。在本章中,我们描述了用聚合酶链反应(PCR)筛选啮齿动物组织中新型冠状病毒的过程,该方法易于适用于一系列动物的筛选。