Kivistö Ilkka, Tidenberg Eeva-Maria, Lilley Thomas, Suominen Kati, Forbes Kristian M, Vapalahti Olli, Huovilainen Anita, Sironen Tarja
Department of Virology, University of Helsinki, Helsinki, Finland.
Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland.
Vector Borne Zoonotic Dis. 2020 Feb;20(2):155-158. doi: 10.1089/vbz.2018.2367. Epub 2019 Sep 10.
Coronaviruses (CoVs) represent a global public health threat, exemplified by the severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) outbreaks. Using fecal samples collected from five bat species between 2014 and 2016 in Finland and RT-PCR, RT-qPCR, and NGS, we identified CoVs in 10 of 79 (13%) samples, including two novel bat species-CoV relationships. Phylogenetic analysis revealed and species clustered among previously identified bat and human viruses. These results expand the known northern distribution and host species range of bat-borne CoVs.
冠状病毒（CoVs）构成全球公共卫生威胁，严重急性呼吸综合征（SARS）和中东呼吸综合征（MERS）疫情就是例证。我们利用2014年至2016年期间在芬兰从五种蝙蝠物种采集的粪便样本，通过逆转录聚合酶链反应（RT-PCR）、逆转录定量聚合酶链反应（RT-qPCR）和下一代测序（NGS），在79个样本中的10个（13%）样本中鉴定出冠状病毒，包括两种新型蝙蝠物种与冠状病毒的关系。系统发育分析显示，[具体物种名称1]和[具体物种名称2]物种聚集在先前鉴定的蝙蝠和人类病毒之中。这些结果扩大了已知的蝙蝠传播冠状病毒在北方的分布范围和宿主物种范围。
