From the National Institute for Cellular Biotechnology, Dublin City University.
Department of Hepatobiliary and Liver Transplant Surgery, St. Vincent's University Hospital.
Pancreas. 2020 Sep;49(8):1109-1116. doi: 10.1097/MPA.0000000000001633.
A limited repertoire of good pancreatic ductal adenocarcinoma (PDAC) models is one of the main barriers in developing effective new PDAC treatments. We aimed to characterize 6 commonly used PDAC cell lines and compare them with PDAC patient tumor samples using proteomics.
Proteomic methods were used to generate an extensive catalog of proteins from 10 PDAC surgical specimens, 9 biopsies of adjacent normal tissue, and 6 PDAC cell lines. Protein lists were interrogated to determine what extent the proteome of the cell lines reflects the proteome of primary pancreatic tumors.
We identified 7973 proteins from the cell lines, 5680 proteins from the tumor tissues, and 4943 proteins from the adjacent normal tissues. We identified 324 proteins unique to the cell lines, some of which may play a role in survival of cells in culture. Conversely, a list of 63 proteins expressed only in the patient samples, whose expression is lost in culture, may place limitations on the degree to which these model systems reflect tumor biology in vivo.
Our work offers a catalog of proteins detected in each of the PDAC cell lines, providing a useful guide for researchers seeking model systems for PDAC functional studies.
良好的胰腺导管腺癌 (PDAC) 模型数量有限，这是开发有效新 PDAC 治疗方法的主要障碍之一。我们旨在使用蛋白质组学方法对 6 种常用的 PDAC 细胞系进行特征描述，并将其与 PDAC 患者肿瘤样本进行比较。
使用蛋白质组学方法从 10 个 PDAC 手术标本、9 个相邻正常组织活检和 6 个 PDAC 细胞系中生成广泛的蛋白质目录。对蛋白质列表进行查询，以确定细胞系的蛋白质组在多大程度上反映了原发性胰腺肿瘤的蛋白质组。
我们从细胞系中鉴定出 7973 种蛋白质，从肿瘤组织中鉴定出 5680 种蛋白质，从相邻正常组织中鉴定出 4943 种蛋白质。我们鉴定出 324 种仅存在于细胞系中的蛋白质，其中一些可能在细胞培养中的存活中发挥作用。相反，63 种仅在患者样本中表达而在培养中丢失的蛋白质列表，可能限制了这些模型系统在多大程度上反映体内肿瘤生物学。
我们的工作提供了每种 PDAC 细胞系中检测到的蛋白质目录，为寻求 PDAC 功能研究模型系统的研究人员提供了有用的指南。
