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藻类趋化性的定量测定。

Quantitative assay for algal chemotaxis.

作者信息

Sjoblad R D, Chet I, Mitchell R

出版信息

Appl Environ Microbiol. 1978 Dec;36(6):847-50. doi: 10.1128/aem.36.6.847-850.1978.

DOI:10.1128/aem.36.6.847-850.1978
PMID:32834
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243156/
Abstract

A quantitative capillary assay is described for measuring chemoreception in the neritic and littoral unicellular alga Dunaliella tertiolecta. Lucite chemotaxis plates were used in the assay with 3-microliter capillaries. A Coulter Counter was employed to determine algal cell numbers. D. tertiolecta is attracted to ammonium ion with a maximum positive response at 10(-3) M. Inclusion of calcium and L-methionine in the chemotaxis medium stimulates algal chemoreception, although neither chemical is essential for motility. Attraction of the chlorophyte to ammonium is dependent on time of incubation, cell density, and pH. The optimum pH for attraction was found to be 6.25.

摘要

描述了一种定量毛细管测定法，用于测量浅海和滨海单细胞藻类杜氏盐藻（Dunaliella tertiolecta）的化学感受。该测定使用了有机玻璃趋化性平板和3微升的毛细管。采用库尔特计数器来确定藻类细胞数量。杜氏盐藻对铵离子有吸引力，在10^(-3) M时产生最大正向反应。趋化性培养基中加入钙和L-甲硫氨酸可刺激藻类的化学感受，尽管这两种化学物质对于运动性都不是必需的。绿藻对铵的吸引力取决于孵育时间、细胞密度和pH值。发现吸引的最佳pH值为6.25。

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本文引用的文献

1
Chloroplast pigments of the marine dinoflagellateGyrodinium resplendens.海洋双鞭毛藻华丽陀螺藻的叶绿体色素
Lipids. 1968 Jan;3(1):5-13. doi: 10.1007/BF02530961.
2
Chemoreceptors in bacteria.细菌中的化学感受器
Science. 1969 Dec 26;166(3913):1588-97. doi: 10.1126/science.166.3913.1588.
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Displacement into incubation medium by albumin of highly unsaturated retina free fatty acids arising from membrane lipids.
FEBS Lett. 1974 Mar 15;40(1):53-6. doi: 10.1016/0014-5793(74)80892-6.
4
A response regulator model in a simple sensory system.一个简单感觉系统中的响应调节模型。
Science. 1977 Jun 3;196(4294):1055-63. doi: 10.1126/science.870969.
5
Chamber for bacterial chemotaxis experiments.用于细菌趋化性实验的小室。
Appl Environ Microbiol. 1976 Nov;32(5):729-30. doi: 10.1128/aem.32.5.729-730.1976.
6
Calcium ion regulates chemotactic behaviour in bacteria.
Nature. 1977 Nov 3;270(5632):66-7. doi: 10.1038/270066a0.
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Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins.大肠杆菌中的感觉转导：涉及甲基化蛋白的两种互补信息处理途径。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3312-6. doi: 10.1073/pnas.74.8.3312.