莱茵衣藻的趋化反应。
Chemotactic responses of Chlamydomonas reinhardtii.
作者信息
Sjoblad R D, Frederikse P H
出版信息
Mol Cell Biol. 1981 Dec;1(12):1057-60. doi: 10.1128/mcb.1.12.1057-1060.1981.
Abstract
A capillary chemotaxis assay revealed that among a wide range of inorganic and organic chemicals, only ammonium ion (NH4+) could serve as an attractant of Chlamydomonas reinhardtii. NH4+ (10(-2) M) gave the maximum response, with up to a 15-fold increase in accumulated algae being measured. No repellents for the chlorophyte were detected. The response to NH4+ was influenced by exogenous levels of calcium, but not by L-methionine. The optimal pH for positive chemotaxis was 7.0; however, attraction was measurable from pH 4.0 to 9.0. Positive chemotaxis was stimulated by performing the assay under fluorescent illumination rather than in the dark.
摘要
毛细管趋化性分析表明，在众多无机和有机化学物质中，只有铵离子（NH4+）可作为莱茵衣藻的引诱剂。10(-2) M的NH4+产生最大反应，测得积累的藻类最多增加15倍。未检测到对绿藻的驱避剂。对NH4+的反应受外源钙水平影响，但不受L-甲硫氨酸影响。正向趋化性的最佳pH值为7.0；然而，在pH 4.0至9.0之间均可检测到吸引作用。在荧光照明而非黑暗条件下进行该分析可刺激正向趋化性。
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