谷胱甘肽与真菌代谢产物镰刀菌素C的化学及酶促相互作用。

The chemical and enzymatic interaction of glutathione with the fungal metabolite, fusarin C.

作者信息

Gelderblom W C, Thiel P G, van der Merwe K J

机构信息

Research Institute for Nutritional Diseases, South African Medical Research Council, Tygerberg.

出版信息

Mutat Res. 1988 May;199(1):207-14. doi: 10.1016/0027-5107(88)90247-3.

DOI:10.1016/0027-5107(88)90247-3

PMID:3283546

Abstract

Glutathione (GSH) interacts both chemically and enzymatically with fusarin C, a mutagenic metabolite produced by Fusarium moniliforme. The chemical reaction, which is pH-dependent, results in the formation of both fusarin A and a compound that lacks the 2-pyrrolidone moiety thereby suggesting an interaction at the C-13-C-14 epoxide. Enzymatic interaction of fusarin C with GSH also appears to occur at this site as fusarins A and D, which lack the epoxide, do not serve as substrates for GSH-S-transferases. The interaction of GSH with fusarin C appears to be an important deactivation step which could explain the lack of carcinogenicity observed for fusarin C in rats.

摘要

谷胱甘肽（GSH）与串珠镰刀菌产生的诱变代谢产物镰刀菌素C发生化学和酶促相互作用。这种化学反应依赖于pH值，会导致镰刀菌素A和一种缺乏2-吡咯烷酮部分的化合物的形成，从而表明在C-13-C-14环氧化物处发生了相互作用。镰刀菌素C与GSH的酶促相互作用似乎也发生在这个位点，因为缺乏环氧化物的镰刀菌素A和D不是GSH-S-转移酶的底物。GSH与镰刀菌素C的相互作用似乎是一个重要的失活步骤，这可以解释在大鼠中观察到的镰刀菌素C缺乏致癌性的现象。