National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK; London Centre for Nanotechnology, University College London, London WC1H 0AH, UK; Department of Physics & Astronomy, University College London, London WC1E 6BT, UK.
National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK; Department of Physics, King's College London, Strand Lane, London WC2R 2LS, UK.
Biochim Biophys Acta Biomembr. 2021 Jan 1;1863(1):183447. doi: 10.1016/j.bbamem.2020.183447. Epub 2020 Aug 21.
Atomic force microscopy is an increasingly attractive tool to study how peptides disrupt membranes. Often performed on reconstituted lipid bilayers, it provides access to time and length scales that allow dynamic investigations with nanometre resolution. Over the last decade, AFM studies have enabled visualisation of membrane disruption mechanisms by antimicrobial or host defence peptides, including peptides that target malignant cells and biofilms. Moreover, the emergence of high-speed modalities of the technique broadens the scope of investigations to antimicrobial kinetics as well as the imaging of peptide action on live cells in real time. This review describes how methodological advances in AFM facilitate new insights into membrane disruption mechanisms.
原子力显微镜是一种越来越有吸引力的工具，可用于研究肽如何破坏膜。它通常在重建的脂质双层上进行，可提供纳米分辨率的动态研究所需的时间和长度尺度。在过去的十年中，原子力显微镜研究使我们能够观察到抗菌肽或宿主防御肽破坏细胞膜的机制，包括靶向恶性细胞和生物膜的肽。此外，该技术的高速模式的出现拓宽了研究范围，包括抗菌动力学以及实时观察肽对活细胞作用的成像。本文综述了原子力显微镜方法学的进展如何促进我们对膜破坏机制的新认识。
