Wang Panliang, Wu Dong, You Xinxin, Su Yinglong, Xie Bing
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China.
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China; Engineering Research Center for Nanophotonics and Advanced Instrument, Ministry of Education, East China Normal University, Shanghai, 200062, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China.
J Hazard Mater. 2021 Feb 5;403:123689. doi: 10.1016/j.jhazmat.2020.123689. Epub 2020 Aug 15.
Landfilled antibiotics and metals were related to the occurrences of their resistance genes, whose decade-long development in leachates with the dynamic landfilling environmental conditions, especially with the varying nitrogen contents, has yet to be studied. Here, we sampled leachates from five representative municipal solid waste landfills in China. The total concentrations of antibiotics (5000 - 50000 ng/L) and metals (10 - 60 mg/L) in leachates were significantly different among different sites and they were only closely related to sulfonamide and tetracycline resistance genes (P < 0.05). Regarding the abundance of subtype resistance genes, sul1 and ermB were dominant antibiotic resistance genes (ARGs) and terc, arsc, and mer were dominant heavy metal resistance genes (HMRGs); and meanwhile the observed huge variations of these genes appeared to be related to environmental factors like nitrate and pH (P < 0.05). The GeoChip results further indicated that more than 85% of sequenced ARGs/HMRGs and nitrogen processing genes, particularly of the denitrification genes, were hosted by the same bacterial species, such as Pseudomonas sp. and Bacillus sp., which belonged to the predominant phylum in leachates. These results extended our knowledge about the linkages among ARGs, HMRGs and nitrogen-processing functions in leachates.
填埋的抗生素和金属与它们抗性基因的出现有关，而这些抗性基因在渗滤液中随动态填埋环境条件（尤其是不同的氮含量）长达十年的发展情况尚未得到研究。在此，我们采集了中国五个具有代表性的城市固体废弃物填埋场的渗滤液样本。不同填埋场渗滤液中抗生素（5000 - 50000纳克/升）和金属（10 - 60毫克/升）的总浓度存在显著差异，且它们仅与磺胺类和四环素抗性基因密切相关（P < 0.05）。关于亚型抗性基因的丰度，sul1和ermB是主要的抗生素抗性基因（ARGs），而terc、arsc和mer是主要的重金属抗性基因（HMRGs）；同时，这些基因所观察到的巨大差异似乎与硝酸盐和pH值等环境因素有关（P < 0.05）。基因芯片结果进一步表明，超过85%的已测序ARGs/HMRGs和氮处理基因，特别是反硝化基因，由相同的细菌物种携带，如假单胞菌属和芽孢杆菌属，它们属于渗滤液中的优势菌门。这些结果扩展了我们对渗滤液中ARGs、HMRGs和氮处理功能之间联系的认识。
