利用工程化的硫嗜碱菌提高确认的纳米级硫生物产量。
Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus.
机构信息
Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences (CAS), Beijing 100190, China; College of Chemical Engineering, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing 100049, China.
Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences (CAS), Beijing 100190, China; College of Chemical Engineering, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing 100049, China; Processes Design and Development Department, Egyptian Petroleum Research Institute, Nasr City, Cairo 11727, Egypt.
出版信息
Bioresour Technol. 2020 Dec;317:124018. doi: 10.1016/j.biortech.2020.124018. Epub 2020 Aug 14.
Complicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25-40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric (>50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus.
复杂的生产工艺和纳米级硫磺的优越特性使其价格比微级硫磺高出 25-40 倍。此外，由于其具有毒性的环境后果，天然气中的硫化氢含量受到限制。硫烷菌属（Thioalkalivibrio versutus）是一种具有高天然气脱硫能力的多极端工业自养生物。在这里，验证了使用 T. versutus 在脱硫天然气的同时生产纳米级（>50nm）硫磺。此外，通过将硫酸盐产量降低 55.1%，将产量提高了 166.7%。特别开发的 CRISPR 系统具有 42%的编辑效率，简化了针对这种具有挑战性的细菌的基因组编辑工作流程方案。同时，通过蛋白质挖掘和转录组研究揭示了硫磺代谢，以确定产生硫酸盐的关键基因（异二硫键还原酶样复合物、硫磺双加氧酶、亚硫酸脱氢酶和亚硫酸氧化酶）。本研究提供了具有成本效益的纳米级硫磺生产，并使用新型 CRISPR 策略提高了这种生产效率，这可能适合于工业多极端微生物，同时揭示了 T. versutus 中的硫磺途径。
Zotero 插件