CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, 050018, Hebei, China.
Arch Microbiol. 2022 May 14;204(6):317. doi: 10.1007/s00203-022-02925-7.
Bacteria are important participants in sulfur cycle of the extremely haloalkaline environment, e.g. soda lake. The effects of physicochemical factors on the composition of sulfide-oxidizing bacteria (SOB) and sulfate-reducing bacteria (SRB) in soda lake have remained elusive. Here, we surveyed the community structure of total bacteria, SOB and SRB based on 16S rRNA, soxB and dsrB gene sequencing, respectively, in five soda lakes with different physicochemical factors. The results showed that the dominant bacteria belonged to the phyla Proteobacteria, Bacteroidetes, Halanaerobiaeota, Firmicutes and Actinobacteria. SOB and SRB were widely distributed in lakes with different physicochemical characteristics, and the community composition were different. In general, salinity and inorganic nitrogen sources (NH-N, NO-N) were the most significant factors. Specifically, the communities of SOB, mainly including Thioalkalivibrio, Burkholderia, Paracoccus, Bradyrhizobium, and Hydrogenophaga genera, were remarkably influenced by the levels of NH-N and salinity. Yet, for SRB communities, including Desulfurivibrio, Candidatus Electrothrix, Desulfonatronospira, Desulfonatronum, Desulfonatronovibrio, Desulfonatronobacter and so on, the most significant determinants were salinity and NO-N. Besides, Rhodoplanes played a significant role in the interaction between SOB and SRB. From our results, the knowledge regarding the community structures of SOB and SRB in extremely haloalkaline environment was extended.
细菌是极端嗜碱环境（如苏打湖）硫循环的重要参与者。然而，理化因素对苏打湖中硫化物氧化菌（SOB）和硫酸盐还原菌（SRB）组成的影响仍不清楚。本研究基于 16S rRNA、soxB 和 dsrB 基因测序，分别调查了五个具有不同理化因子的苏打湖中总细菌、SOB 和 SRB 的群落结构。结果表明，优势细菌属于变形菌门、拟杆菌门、盐厌氧菌门、厚壁菌门和放线菌门。SOB 和 SRB 广泛分布于具有不同理化特征的湖泊中，其群落组成也存在差异。总的来说，盐度和无机氮源（NH4-N、NO3-N）是最重要的因素。具体来说，SOB 群落，主要包括硫碱杆菌属、伯克霍尔德氏菌属、副球菌属、慢生根瘤菌属、噬氢菌属等，受 NH4-N 和盐度水平的显著影响。然而，对于 SRB 群落，包括脱硫弧菌属、电硫菌属、脱硫贝氏硫杆菌属、脱硫硝杆菌属、脱硫硝球菌属、脱硫反硝化杆菌属等，最重要的决定因素是盐度和 NO3-N。此外，红平菌属在 SOB 和 SRB 之间的相互作用中发挥了重要作用。本研究结果扩展了对极端嗜碱环境中 SOB 和 SRB 群落结构的认识。
