Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
J Med Microbiol. 2020 Sep;69(9):1151-1168. doi: 10.1099/jmm.0.001246. Epub 2020 Aug 25.
is a facultative, anaerobic, opportunistic pathogen associated with medical and dental diseases. Bacterial phenotypic traits and pathogenesis are often influenced by lysogeny. The aim of this study was to characterize both the morphology and complete genome sequences of induced prophages purified from clinical isolates. isolates were recovered from the roots of teeth of patients attending an endodontic clinic. The morphological features of isolated phage were characterized using transmission electron microscopy (TEM). DNA sequencing was performed using the Illumina MiSeq platform. TEM indicated that the isolated φEf-vB1 prophage belongs to the family . The φEf-vB1 prophage was stable over a wide range of temperatures and pH. Sequencing of φEf-vB1 DNA revealed that the phage genome is 37 561 bp in length with a G+C content of 37.6mol% and contained 53 ORFs. Comparison with previously predicted prophage genomes using blast revealed that φEf-vB1 has a high sequence similarity to previously characterized phage genomes. The lysogenic strain exhibited a higher biofilm formation capacity relative to the non-lysogenic strain. The current findings highlight the role of lysogeny in modification of properties and reveal the potential importance of prophages in biology and pathogenesis.
是一种兼性厌氧、机会性病原体，与医学和牙科疾病有关。细菌表型特征和发病机制通常受溶原性影响。本研究旨在从临床分离株中纯化诱导前噬菌体的形态和完整基因组序列进行特征描述。从接受牙髓病学诊所治疗的患者的牙齿根部回收 分离株。使用透射电子显微镜 (TEM) 对分离噬菌体的形态特征进行了表征。使用 Illumina MiSeq 平台进行 DNA 测序。TEM 表明，分离的 φEf-vB1 噬菌体属于 科。φEf-vB1 噬菌体在很宽的温度和 pH 范围内稳定。测序表明 φEf-vB1 DNA 长 37561bp，G+C 含量为 37.6mol%，包含 53 个 ORF。与使用 blast 预测的先前预测的噬菌体基因组进行比较表明，φEf-vB1 与以前表征的噬菌体基因组具有很高的序列相似性。溶原性菌株相对于非溶原性菌株表现出更高的生物膜形成能力。目前的研究结果强调了溶原性在修饰 特性中的作用，并揭示了前噬菌体在 生物学和发病机制中的潜在重要性。
