School of Engineering, College of Engineering and Physical Sciences, University of Guelph, Guelph, ON, Canada.
Electrical Engineering, Federal Institute of ParaÍba, João Pessoa, PB, Brazil.
Electrophoresis. 2020 Dec;41(23):1961-1968. doi: 10.1002/elps.202000116. Epub 2020 Sep 6.
This paper presents an inexpensive and easy-to-implement voltage sequencer instrument for use in microchip capillary electrophoresis (MCE) actuation. The voltage sequencer instrument takes a 0-5 V input signal from a microcontroller and produces a reciprocally proportional voltage signal with the capability to achieve the voltages required for MCE actuation. The unit developed in this work features four independent voltage channels, measures 105 × 143 × 45 mm (width × length × height), and the cost to assemble is under 60 USD. The system is controlled by a peripheral interface controller and commands are given via universal serial bus connection to a personal computer running a command line graphical user interface. The performance of the voltage sequencer is demonstrated by its integration with a fluorescence spectroscopy MCE sensor using pinched sample injection and electrophoretic separation to detect ciprofloxacin in samples of milk. This application is chosen as it is particularly important for the dairy industry, where fines and health concerns are associated with the shipping of antibiotic-contaminated milk. The voltage sequencer instrument presented represents an effective low-cost instrumentation method for conducting MCE, thereby making these experiments accessible and affordable for use in industries such as the dairy industry.
本文提出了一种廉价且易于实现的微芯片毛细管电泳（MCE）驱动用电压序列器仪器。该电压序列器仪器从微控制器接收 0-5V 输入信号，并产生与之成比例的电压信号，能够实现 MCE 驱动所需的电压。本工作中开发的单元具有四个独立的电压通道，尺寸为 105×143×45mm（宽×长×高），组装成本低于 60 美元。该系统由外围接口控制器控制，并通过通用串行总线连接到个人计算机上的命令行图形用户界面发出命令。通过使用夹流进样和电泳分离将其与荧光光谱 MCE 传感器集成，该电压序列器用于检测牛奶样品中的环丙沙星，从而展示了其性能。选择此应用程序是因为它对于乳制品行业特别重要，在乳制品行业中，与运输抗生素污染的牛奶相关的罚款和健康问题令人关注。所提出的电压序列器仪器代表了一种有效的低成本 MCE 仪器方法，从而使这些实验在乳制品等行业中易于使用且价格合理。
