Leclerc Camille A, Ty Christopher G D, Worthington Sean S, Richardson Malley B, AlSawalhi Abdulla K, Wood Larry, Moomand Khalid, Collier Christopher M
Collier Research Group, School of Engineering, The University of British Columbia, Kelowna, British Columbia, Canada.
CG Wellington, Drayton, Ontario, Canada.
Electrophoresis. 2025 Feb;46(3-4):143-151. doi: 10.1002/elps.202400079. Epub 2025 Feb 13.
This work describes a cross-shaped PMMA ME system capable of detecting ciprofloxacin hydrochloride monohydrate (CPFH) in unfiltered milk samples. The cross-shaped PMMA ME system utilizes a BGE consisting primarily of the surface-active agent SDS to solubilize milk fat and improve the zeta potential of the PMMA microchannel surface. A theoretical lumped-element circuit model for cross-shaped ME is introduced in this work to calculate the migration time of CPFH. This manuscript improves the capabilities of PMMA-based ME for CPFH in milk using an SDS-based BGE. The presented ME system has a faster migration time, higher mean output voltage, and thinner full-width at half-maximum than previously reported dairy-based biosensor systems. Most notably, the migration time of the new system is under 10 min, being the time associated with the milking of cattle. The system is also found to be able to detect the presence of milk fat. Discussion is included of potential future integration with existing high-sensitivity methodologies to place the overall ME system's limit of detection below an established target. To the authors' knowledge, this is the first reported account of a PMMA ME system capable of detecting CPFH in unfiltered milk.
这项工作描述了一种十字形聚甲基丙烯酸甲酯微流控电泳（PMMA ME）系统，该系统能够检测未经过滤的牛奶样品中的盐酸环丙沙星一水合物（CPFH）。十字形PMMA ME系统利用主要由表面活性剂十二烷基硫酸钠（SDS）组成的背景电解质（BGE）来溶解乳脂肪，并改善PMMA微通道表面的zeta电位。本文引入了一种用于十字形微流控电泳的理论集总元件电路模型，以计算CPFH的迁移时间。本论文利用基于SDS的BGE提高了基于PMMA的微流控电泳检测牛奶中CPFH的能力。所展示的微流控电泳系统具有比先前报道的基于乳制品的生物传感器系统更快的迁移时间、更高的平均输出电压和更窄的半高宽。最值得注意的是，新系统的迁移时间在10分钟以内，这与奶牛挤奶的时间相关。该系统还被发现能够检测乳脂肪的存在。文中还讨论了未来与现有高灵敏度方法进行整合的可能性，以使整个微流控电泳系统的检测限低于既定目标。据作者所知，这是首次报道能够检测未经过滤牛奶中CPFH的PMMA微流控电泳系统。
