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Electron microscopic studies on human basophils from atopic and nonatopic subjects, using horse radish peroxidase labelled anti-IgE.

作者信息

van Elven E H, Stallman P J, Brühl P C

出版信息

Int Arch Allergy Appl Immunol. 1977;54(6):560-7. doi: 10.1159/000231877.

DOI:10.1159/000231877
PMID:328411
Abstract

In previous studies, quantitative differences in cell-bound IgE on the basophils from various donors were measured by means of a quantitative immunofluorescence technique. In this study, cell-bound IgE was demonstrated on basophilic granulocytes by immunoelectron microscopy, using horse radish peroxidase labelled anti-IgE. The distribution of IgE on the basophils was studied in both atopic and nonatopic subjects. The intensity of the peroxidase staining on the basophils varied and appeared to correlate with the amount of cell-bound IgE, as estimated by quantitative immunofluorescence.

摘要

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