Diel F, Detscher M, Schock B, Ennis M
Department of Nutrition, University of Applied Sciences Fulda, Germany.
Allergy. 1998 Nov;53(11):1052-9. doi: 10.1111/j.1398-9995.1998.tb03814.x.
Synthetic pyrethroids are increasingly used as insecticides and marketed as having relatively low human toxicity. The aim of this study was to examine the in vitro effects of the synthetic pyrethroid S-bioallethrin on human blood lymphocytes and basophils in atopic individuals and nonatopic control subjects. S-bioallethrin caused inhibition of lymphocyte proliferation after a 72-h culture period in a concentration-dependent manner. The inhibition of the lymphocyte proliferation by S-bioallethrin at the concentration 6.5 microM correlated well with the total serum IgE values (r = -0.89, P < 0.001). Samples from atopic subjects were more sensitive to this inhibition than those from nonatopic volunteers. The regulatory interleukin-4/interferon-gamma (JL-4/IFN-gamma) balance showed a significant difference between atopic and nonatopic subjects after a short-term culture period (24 h) in the presence of the same concentration range of S-bioallethrin (P < 0.001). Additionally, IFN-gamma secretion was consistently lower in cells from the atopic donors. Furthermore, S-bioallethrin induced histamine release from human basophils in a concentration-dependent manner. Although the effect was small compared to histamine liberators such as N-formyl-Met-Leu-Phe and anti-IgE, the response to S-bioallethrin was significantly different in atopic donors from nonatopic (P = 0.0431). These findings are the first demonstration of the immunotoxicologic properties of the synthetic pyrethroid S-bioallethrin by this combined in vitro approach with human lymphocytes and basophils. Further studies will investigate the responses of lymphocytes from patients who are sensitive to these agents.
合成拟除虫菊酯越来越多地被用作杀虫剂，并以对人体毒性相对较低进行销售。本研究的目的是检测合成拟除虫菊酯S - 生物烯丙菊酯对特应性个体和非特应性对照受试者的人血淋巴细胞和嗜碱性粒细胞的体外作用。在72小时培养期后，S - 生物烯丙菊酯以浓度依赖性方式抑制淋巴细胞增殖。6.5微摩尔浓度的S - 生物烯丙菊酯对淋巴细胞增殖的抑制作用与血清总IgE值密切相关（r = -0.89，P < 0.001）。来自特应性受试者的样本比来自非特应性志愿者的样本对这种抑制更敏感。在相同浓度范围的S - 生物烯丙菊酯存在下短期培养（24小时）后，特应性和非特应性受试者之间的调节性白细胞介素-4/干扰素-γ（IL - 4/IFN - γ）平衡显示出显著差异（P < 0.001）。此外，来自特应性供体的细胞中IFN - γ分泌始终较低。此外，S - 生物烯丙菊酯以浓度依赖性方式诱导人嗜碱性粒细胞释放组胺。尽管与组胺释放剂如N - 甲酰 - 甲硫氨酰 - 亮氨酰 - 苯丙氨酸和抗IgE相比，这种作用较小，但特应性供体对S - 生物烯丙菊酯的反应与非特应性供体有显著差异（P = 0.0431）。这些发现首次通过这种将人淋巴细胞和嗜碱性粒细胞结合的体外方法证明了合成拟除虫菊酯S - 生物烯丙菊酯的免疫毒理学特性。进一步的研究将调查对这些药物敏感的患者淋巴细胞的反应。
