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[甲醇营养型酵母代谢产物导致培养基酸化的本质]

[The nature of methanol-induced acidification of the medium by products of metabolism of methylotrophic yeasts].

作者信息

Gonchar M V, Sibirnyĭ A A

出版信息

Ukr Biokhim Zh (1978). 1988 Jan-Feb;60(1):97-100.

PMID:3284121
Abstract

The washed cells of Hansenula polymorpha and Pichia pinus grown on the medium with methanol rapidly acidify the medium during incubation with the mentioned alcohol or formaldehyde. It is found that proton extrusion is coupled with formate anion efflux. Acidification is proved to be energy-dependent process since it is inhibited by respiration poisons, uncouplers of oxidative phosphorylation, and by ATPase inhibitors.

摘要

在以甲醇为培养基生长的多形汉逊酵母和松毕赤酵母的洗涤细胞,在与上述醇类或甲醛一起孵育期间会迅速使培养基酸化。发现质子外排与甲酸根阴离子外流相关联。酸化被证明是一个能量依赖的过程,因为它受到呼吸抑制剂、氧化磷酸化解偶联剂和ATP酶抑制剂的抑制。

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