Murdanoto A P, Sakai Y, Sembiring L, Tani Y, Kato N
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Biosci Biotechnol Biochem. 1997 Aug;61(8):1391-3. doi: 10.1271/bbb.61.1391.
A water-soluble ester, methyl formate, was detected as a metabolite in the culture medium of methylotrophic yeasts. Methyl formate synthase, which catalyses NAD(+)-dependent dehydrogenation of the hemiacetal adduct of methanol and formaldehyde, catalyses the ester synthesis. The enzyme activity was induced on a methanol medium and was increased further by the addition of formaldehyde. In the reaction system using intact cells of Pichia methanolica AKU 4262, 135 mM (8.1 g/liter) methyl formate was produced from 2 M methanol. This is a new biological process for ester synthesis that couples spontaneous formation of hemiacetal and alcohol dehydrogenase.
甲酸甲酯作为一种水溶性酯，在甲基营养型酵母的培养基中被检测为代谢产物。催化甲醇和甲醛半缩醛加合物的NAD(+)依赖性脱氢反应的甲酸甲酯合酶催化酯的合成。该酶活性在甲醇培养基上被诱导，添加甲醛后进一步增强。在使用甲醇毕赤酵母AKU 4262完整细胞的反应体系中，由2 M甲醇产生了135 mM（8.1 g/升）的甲酸甲酯。这是一种将半缩醛的自发形成与醇脱氢酶偶联的酯合成新生物过程。
