Lin Lin, Dai Fei, Wei Jinjin, Chen Zheng
Department of Otorhinolaryngology Head and Neck Surgery，Huashan Hospital North of Fudan University，Shanghai，201907，China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2020 Aug;34(8):701-705. doi: 10.13201/j.issn.2096-7993.2020.08.006.
CD8⁺CD25⁺Foxp3⁺regulatory T（Treg） cells are reduced in chronic rhinosinusitis with nasal polyps（CRSwNP）. However, the role of these cells in nasal polyps（NP） has not been fully investigated. The aim of this study was to evaluate the influence of budesonide treatment on these cells and to assess their roles in neutrophilic NP. Eight neutrophilic CRSwNP patients were enrolled and received budesonide nasal spray treatment for three months. Nasal samples were obtained from normal mucosa or NP and cultured in vitro. CD8⁺CD25⁺Foxp3⁺Treg cells were isolated and purified from normal or NP tissues and cultured in vitro. Then transforming growth factor-β（TGF-β） and its mRNA were examined in those cell cultures. After that, those cells were administered into NP cultures. Finally, the concentrations of myeloperoxidase（MPO） and interferon（IFN） -γ were evaluated in those tissue cultures. CD8⁺CD25⁺Foxp3⁺Treg cells were decreased in NP compared to normal tissues. Budesonide treatment did not increase the percentage of those cells in NP. TGF-β and its mRNA were enhanced in CD8⁺CD25⁺Foxp3⁺Treg cell cultures from NP versus those from normal tissues. In addition, levels of MPO and IFN-γ were reduced after CD8⁺CD25⁺Foxp3⁺Treg cells administration. These findings indicated that CD8⁺CD25⁺Foxp3⁺Treg cells may play a role in the regulation of neutrophilic inflammation, and budesonide treatment may not influence these Treg cells.
在伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）中，CD8⁺CD25⁺Foxp3⁺调节性T（Treg）细胞数量减少。然而，这些细胞在鼻息肉（NP）中的作用尚未得到充分研究。本研究旨在评估布地奈德治疗对这些细胞的影响，并评估它们在嗜中性粒细胞性NP中的作用。招募了8例嗜中性粒细胞性CRSwNP患者，给予布地奈德鼻喷雾剂治疗3个月。从正常黏膜或NP获取鼻样本并进行体外培养。从正常或NP组织中分离并纯化CD8⁺CD25⁺Foxp3⁺Treg细胞，然后进行体外培养。接着检测这些细胞培养物中的转化生长因子-β（TGF-β）及其mRNA。之后，将这些细胞加入到NP培养物中。最后，评估这些组织培养物中髓过氧化物酶（MPO）和干扰素（IFN）-γ的浓度。与正常组织相比，NP中的CD8⁺CD25⁺Foxp3⁺Treg细胞减少。布地奈德治疗并未增加NP中这些细胞的百分比。与来自正常组织的CD8⁺CD25⁺Foxp3⁺Treg细胞培养物相比，NP来源的培养物中TGF-β及其mRNA水平升高。此外，加入CD8⁺CD25⁺Foxp3⁺Treg细胞后，MPO和IFN-γ水平降低。这些发现表明，CD8⁺CD25⁺Foxp3⁺Treg细胞可能在嗜中性粒细胞性炎症的调节中发挥作用，且布地奈德治疗可能不会影响这些Treg细胞。
