不同抗体检测方法检测水痘-带状疱疹病毒的敏感性和特异性。
Sensitivity and specificity of different antibody tests for detecting varicella-zoster virus.
作者信息
Otani Naruhito, Shima Masayuki, Tanimura Susumu, Ueda Takashi, Ichiki Kaoru, Nakajima Kazuhiko, Takesue Yoshio, Honjo Kenta, Okuno Toshiomi
机构信息
Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan.
Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan.
出版信息
J Infect Chemother. 2020 Dec;26(12):1283-1287. doi: 10.1016/j.jiac.2020.07.012. Epub 2020 Aug 22.
INTRODUCTION
Antibody tests for detecting varicella-zoster virus include the fluorescent-antibody-to-membrane-antigen (FAMA) assay, immune adherence hemagglutination assay (IAHA), enzyme immunoassay (EIA), and the glycoprotein-based enzyme-linked immunosorbent assay (gpELISA). Although FAMA and gpELISA are highly sensitive, FAMA is not available commercially. Therefore, this study was performed to compare potential high-sensitivity tests with commercially available tests.
METHODS
Four antibody tests, FAMA, gpELISA, EIA, and IAHA, were performed using sera collected from 32 children aged 7 months-10 years. Using FAMA as a reference, the sensitivity and specificity of gpELISA, EIA, and IAHA were assessed. Subsequently, using gpELISA as a reference, the positive agreement rate of EIA and IAHA was assessed.
RESULTS
On a reference scale with FAMA set at 100%, the sensitivity and specificity of the antibody tests were as follows: gpELISA, 67% and 100%; EIA, 67% and 100%; and IAHA, 47% and 100%, respectively. The positive agreement rates of EIA and IAHA relative to gpELISA were 86% and 64%, respectively.
CONCLUSIONS
gpELISA had a lower positive rate than did FAMA, and showed comparable sensitivity to that of EIA.
引言
用于检测水痘带状疱疹病毒的抗体检测方法包括荧光膜抗原抗体检测（FAMA）、免疫粘附血凝试验（IAHA）、酶免疫测定（EIA）以及基于糖蛋白的酶联免疫吸附测定（gpELISA）。尽管FAMA和gpELISA具有高度敏感性，但FAMA没有商业产品。因此，本研究旨在比较潜在的高敏感性检测方法与市售检测方法。
方法
使用从32名7个月至10岁儿童采集的血清进行FAMA、gpELISA、EIA和IAHA这四种抗体检测。以FAMA作为参考，评估gpELISA、EIA和IAHA的敏感性和特异性。随后，以gpELISA作为参考，评估EIA和IAHA的阳性符合率。
结果
在以FAMA设定为100%的参考标准下，抗体检测的敏感性和特异性如下：gpELISA分别为67%和100%；EIA分别为67%和100%；IAHA分别为47%和100%。EIA和IAHA相对于gpELISA的阳性符合率分别为86%和64%。
结论
gpELISA的阳性率低于FAMA，且显示出与EIA相当的敏感性。
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