Yamada A, Ogino S, Asano Y, Otsuka T, Takahashi M, Baba K, Yabuuchi H
Biken J. 1979 Jun;22(2):55-60.
Four tests for antibody to varicella-zoster (V-Z) virus were compared; these were tests of complement fixation (CF), neutralization (NT), fluorescent antibody to membrane antigen (FAMA) and immune adherence hemagglutination (IAHA). Fifty-two sera from patients with varicella and zoster and from recipients of live varicella vaccine were examined by the 4 tests. The CF test was least sensitive, but the antibody titers by the NT, FAMA and IAHA tests were roughly comparable. The IAHA test was the simplest and fastest to perform, and appeared suitable for routine serological assay to V-Z virus. The correlation between the IAHA antibody titer and susceptibility of individuals to clinical varicella was investigated retrospectively using sera obtained during 2 outbreaks of varicella in an institution for children, where all the unvaccinated children had developed varicella symptoms. Most of the 25 pre-exposure sera from unvaccinated children examined by the IAHA test had tiers of less than 1:2. In contrast, all the 23 sera from vaccinated children who did not develop varicella had detectable antibody titers of 1:2 to 1:64. These results indicate that the IAHA titer reflects the susceptibility or resistance of individuals to clinical varicella.
对四种检测水痘 - 带状疱疹（V - Z）病毒抗体的试验进行了比较；这些试验分别是补体结合试验（CF）、中和试验（NT）、膜抗原荧光抗体试验（FAMA）和免疫粘连血凝试验（IAHA）。用这四种试验检测了52份来自水痘和带状疱疹患者以及水痘活疫苗接种者的血清。补体结合试验敏感性最低，但中和试验、膜抗原荧光抗体试验和免疫粘连血凝试验所测得的抗体滴度大致相当。免疫粘连血凝试验操作最简单、速度最快，似乎适用于水痘 - 带状疱疹病毒的常规血清学检测。利用在一所儿童机构两次水痘暴发期间采集的血清，对免疫粘连血凝试验抗体滴度与个体对临床水痘易感性之间的相关性进行了回顾性研究，该机构所有未接种疫苗的儿童均出现了水痘症状。通过免疫粘连血凝试验检测的25份未接种疫苗儿童暴露前血清中，大多数滴度低于1:2。相比之下，23份未患水痘的接种疫苗儿童的血清中，所有血清的抗体滴度均可检测到，范围为1:2至1:64。这些结果表明，免疫粘连血凝试验滴度反映了个体对临床水痘的易感性或抵抗力。
