Univ-Lyon, CarMeN Laboratory, Inserm U1060, INRAE UMR 1397, INSA Lyon, CENS-ELI Building 165 chemin du Grand Revoyet, Pierre-Bénite, 69310, France.
Laboratoire de Biochimie et de Biologie Moléculaire Grand Est, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, Bron, 69500, France.
Mol Nutr Food Res. 2020 Oct;64(19):e2000694. doi: 10.1002/mnfr.202000694. Epub 2020 Sep 9.
The aim of this study is to examine whether postprandial (PP) triglyceride-rich lipoproteins (TGRL) secreted after a moderate fat intake would activate platelets differently according to their fatty acid (FA) composition.
In a parallel single-blind randomized trial, 30 women with type 2 diabetes are assigned a breakfast containing 20 g lipids from butter versus hazelnut-cocoa spread (HCS) rich in palm oil. Blood samples are collected at fasting and 4 h PP. FA composition of fasting and PP TGRL and their effects on the activation of platelets from healthy blood donors are assessed. Both breakfasts similarly increase plasma ApoB-48, plasma, and TGRL triglycerides (p < 0.05). TGRL mean diameter increases after both breakfasts and is greater after the butter breakfast. Both breakfasts are rich in palmitic acid, and the HCS breakfast contains 45% oleic acid. TGRL FA composition reflects the dietary FA composition. Pre-incubation of platelets with fasting and PP TGRL increases collagen-stimulated aggregation (p < 0.01 vs control). Fasting and PP TGRL similarly increase agonist-induced thromboxane B concentrations, and this effect is concentration-dependent for PP TGRL.
PP TGRL from type 2 diabetic women after a palm-oil spread versus butter-based mixed meal induce similar acute in vitro platelet activation.
本研究旨在探究在摄入适量脂肪后，餐后富含甘油三酯的脂蛋白（TGRL）根据其脂肪酸（FA）组成是否会以不同的方式激活血小板。
在一项平行的单盲随机试验中，30 名 2 型糖尿病女性被分配食用含有 20 克黄油脂质的早餐或富含棕榈油的榛子-可可酱（HCS）早餐。空腹和餐后 4 小时采集血液样本。评估空腹和餐后 TGRL 的 FA 组成及其对健康献血者血小板激活的影响。两种早餐均能同等程度地增加血浆 ApoB-48、血浆和 TGRL 甘油三酯（p<0.05）。两种早餐后 TGRL 平均直径均增加，且黄油早餐后增加更明显。两种早餐均富含棕榈酸，HCS 早餐含有 45%的油酸。TGRL 的 FA 组成反映了饮食中的 FA 组成。用空腹和餐后 TGRL 预孵育血小板可增加胶原刺激的聚集（p<0.01 与对照组相比）。空腹和餐后 TGRL 均能同等程度地增加激动剂诱导的血栓素 B 浓度，且这种效应与餐后 TGRL 的浓度呈依赖性。
富含棕榈油的 HCS 早餐和富含黄油的混合餐均可诱导 2 型糖尿病女性餐后 TGRL 相似的急性体外血小板激活。
