Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
Solaris National Synchrotron Radiation Centre, Jagiellonian University, Krakow, Poland.
J Biophotonics. 2020 Dec;13(12):e202000252. doi: 10.1002/jbio.202000252. Epub 2020 Sep 18.
Exposure to ionizing radiation significantly affects biochemistry of cancer cells. The effect of irradiation can be divided into two stages, that is, the physicochemical stage and the biological response. Both effects induce different biochemical changes in the cells and should be analyzed as two separate phenomena. Thus, in the current study, Raman spectroscopy of prostate cancer cells fixed before (the physicochemical damage model) and just after (the biological response model) irradiation was undertaken to compare biochemical composition of irradiated cancer cells at both stages. Spectroscopic analysis of the cells was performed separately for cytoplasmic and nuclear regions. Biochemical changes of irradiated cells were analyzed using partial least squares regression (PLSR) method on the basis of the collected Raman spectra. Regression coefficients were therefore used to describe differences and similarities between biochemical composition of cancer cells undergoing the physicochemical stage and biological response. Additionally, PLSR models of both phenomena were compared for linear dose-dependence and a cross prediction.
电离辐射暴露会显著影响癌细胞的生物化学特性。照射的影响可以分为两个阶段，即物理化学阶段和生物反应。这两种效应都会在细胞中引起不同的生化变化，因此应将其分析为两个独立的现象。因此，在本研究中，对固定在照射前（理化损伤模型）和照射后（生物反应模型）的前列腺癌细胞进行了拉曼光谱分析，以比较两个阶段照射后癌细胞的生化组成。分别对细胞质和核区域的细胞进行光谱分析。基于收集的拉曼光谱，使用偏最小二乘回归（PLSR）方法分析照射细胞的生化变化。回归系数用于描述经历理化阶段和生物反应的癌细胞生化组成之间的差异和相似性。此外，还比较了这两种现象的 PLSR 模型的线性剂量依赖性和交叉预测。
