Roman Maciej, Wrobel Tomasz P, Panek Agnieszka, Paluszkiewicz Czeslawa, Kwiatek Wojciech M
Institute of Nuclear Physics Polish Academy of Sciences, Radzikowskiego 152, 31-342 Krakow, Poland.
Solaris National Synchrotron Radiation Centre, Jagiellonian University, Czerwone Maki 98, 30-392, Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jul 5;255:119653. doi: 10.1016/j.saa.2021.119653. Epub 2021 Mar 8.
Modern techniques of radiotherapy such as fractioned radiotherapy require applications of low doses of ionizing radiation (up to 10 Gy) for effective patient treatment. It is, therefore, crucial to understand the response mechanisms in cancer cells irradiated with low (clinical) doses. The cell's response to irradiation depends on a dose and post-irradiation time. Both factors should be considered when studying the influence of ionizing radiation on cancer cells. Thus, in the present study, PC-3 prostate cancer cells were irradiated with clinical doses of X-rays to determine dose- and time-dependent response to the irradiation. Raman spectroscopy and biological methods (MTT and comet assays) were applied for the analysis of biochemical changes in the cells induced by low doses of X-ray irradiation at 0 h and 24 h post-irradiation timepoints. Due to a limited view of the biochemical changes at the subcellular level given by single spectrum Raman measurements, Raman mapping of the whole cell area was performed. The results were compared with those obtained for cell irradiation with high doses. The analysis was based on the Partial Least Squares Regression (PLSR) method for the cytoplasmic and nuclear regions separately. Additionally, for the first time, irradiation classification was performed to confirm Raman spectroscopy as a powerful tool for studies on cancer cells treated with clinical doses of ionizing radiation.
现代放射治疗技术，如分次放射治疗，需要应用低剂量的电离辐射（高达10 Gy）来有效治疗患者。因此，了解低（临床）剂量照射下癌细胞的反应机制至关重要。细胞对辐射的反应取决于剂量和照射后的时间。在研究电离辐射对癌细胞的影响时，这两个因素都应予以考虑。因此，在本研究中，用临床剂量的X射线照射PC-3前列腺癌细胞，以确定对辐射的剂量和时间依赖性反应。拉曼光谱和生物学方法（MTT和彗星试验）用于分析在照射后0小时和24小时的时间点，低剂量X射线照射诱导的细胞生化变化。由于单光谱拉曼测量对亚细胞水平生化变化的观察有限，因此对整个细胞区域进行了拉曼映射。将结果与高剂量细胞照射所得结果进行比较。分析分别基于细胞质和细胞核区域的偏最小二乘回归（PLSR）方法。此外，首次进行了照射分类，以确认拉曼光谱是研究临床剂量电离辐射治疗癌细胞的有力工具。
