School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
Biomater Sci. 2020 Sep 30;8(19):5230-5240. doi: 10.1039/d0bm00925c.
Enzymatic biofuel cells (EBFCs), as one of the most promising sustainable and green energy sources, have attracted significant interest. However, the limited lifetime and output power of EBFCs deriving from the intrinsic defects of natural enzyme fail to meet the requirements of commercial applications. As a robust approach, protein engineering shows promising potential to overcome these defects. In this review, we will elaborate on the basic principles, structure and electron transfer pathways of EBFCs, and discuss the strategies of protein engineering for improving the performances of EBFCs. We hope that this review will inspire researchers to envisage efficient enzymes for EBFCs and promote the commercial transformation of EBFCs in implantable medical devices, portable power batteries and even clean-power-driven cars in the near future.
酶生物燃料电池（EBFCs）作为最有前途的可持续绿色能源之一，引起了人们的极大关注。然而，由于天然酶的固有缺陷，EBFCs 的有限寿命和输出功率无法满足商业应用的要求。作为一种强大的方法，蛋白质工程显示出克服这些缺陷的巨大潜力。在这篇综述中，我们将详细阐述 EBFCs 的基本原理、结构和电子传递途径，并讨论蛋白质工程策略在提高 EBFCs 性能方面的应用。我们希望这篇综述能激发研究人员设计出高效的 EBFCs 酶，并推动 EBFCs 在植入式医疗器械、便携式电源电池，甚至在清洁动力驱动汽车等领域的商业转化。
