CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Present address: Universidade Católica Portuguesa, Faculty of Dental Medicine, Center for Interdisciplinary Investigation (CIIS), Viseu, Portugal.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5337-5354. doi: 10.1099/ijsem.0.004410. Epub 2020 Aug 26.
Fungi are ubiquitous organisms with a wide distribution in almost all ecosystems, including marine environments. Coastal and estuarine ecosystems remain poorly unexplored as fungal habitats, potentially harbouring a hidden diversity with important ecological roles. During an extensive survey of marine fungi in coastal and estuarine Portuguese environments, a collection of 612 isolates was obtained from water, algae, sponges and driftwood. From these, 282 representative isolates were selected through microsatellite-primed PCR (MSP-PCR) fingerprinting analysis, which were identified based on DNA sequence data. The collection yielded 117 taxa from 38 distinct genera, which were identified using DNA sequence analysis. Overall, fungal community composition varied with host/substrate, but the most abundant taxa in the collection were , , and / complex. The occurrence of a high fungal diversity harbouring novel species was disclosed. Through a multilocus phylogeny based on ITS, and sequences, in conjunction with morphological and physiological data, we propose sp. nov. and sp. nov.
真菌是一种广泛分布于几乎所有生态系统（包括海洋环境）的生物体。沿海和河口生态系统作为真菌栖息地仍未得到充分探索，它们可能蕴藏着具有重要生态作用的隐藏多样性。在对葡萄牙沿海和河口地区海洋真菌的广泛调查中，从水中、藻类、海绵和浮木中获得了 612 个分离物。从中通过微卫星引物 PCR (MSP-PCR) 指纹分析选择了 282 个有代表性的分离物，这些分离物是基于 DNA 序列数据鉴定的。该收集物从 38 个不同的属中鉴定出了 117 个分类单元，这些分类单元是通过 DNA 序列分析鉴定的。总的来说，真菌群落组成随宿主/基质而异，但最丰富的类群是 、 、 和 / 复合体。揭示了蕴藏着新物种的高真菌多样性的存在。通过基于 ITS、 和 序列的多基因系统发育分析，结合形态学和生理学数据，我们提出了 种新种和 种新种。
