The C. Eugene Bennett Department of Chemistry, West Virginia University, 217 Clark Hall, Morgantown, WV, 26506, United States.
The C. Eugene Bennett Department of Chemistry, West Virginia University, 217 Clark Hall, Morgantown, WV, 26506, United States; WVU Rockefeller Neuroscience Institute, West Virginia University, 1 Medical Center Dr., P.O. Box 9303, Morgantown, WV, 26505, United States; Department of Neuroscience, West Virginia University, 1 Medical Center Dr., P.O. Box 9303, Morgantown, WV, 26505, United States.
Anal Biochem. 2020 Nov 15;609:113864. doi: 10.1016/j.ab.2020.113864. Epub 2020 Aug 23.
Mixed polydiacetylene (PDA) lipid vesicles mimic cell membranes and exhibit a colorimetric response induced by mechanical stress, which can be used to determine the affinity of proteins or molecules for lipid membranes. Due to a simple spectroscopic readout, PDA assays are amenable to high-throughput screens; however, these assays exhibit batch-to-batch variability. Sensitivity of the assay is also influenced by physicochemical properties associated with different lipids. Here, a method of normalizing PDA assays to reduce variability and enable direct comparison across lipid systems is described.
混合聚二乙炔（PDA）脂质体模拟细胞膜，并表现出由机械应力诱导的比色响应，可用于测定蛋白质或分子与脂质膜的亲和力。由于具有简单的光谱读出功能，PDA 测定法适用于高通量筛选；但是，这些测定法存在批次间的可变性。测定法的灵敏度还受到与不同脂质相关的物理化学性质的影响。本文描述了一种将 PDA 测定法标准化以减少变异性并能够在不同脂质体系之间进行直接比较的方法。
