Institute of Chemical Sciences, University of Rennes 1, 35000 Rennes, France.
Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, France.
Sensors (Basel). 2018 Feb 15;18(2):599. doi: 10.3390/s18020599.
Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed.
聚二乙炔（PDA）插入薄膜或囊泡中，由于其在受到各种刺激时会发生蓝变红的比色转变，并且从非荧光变为荧光，因此受到越来越多的关注。在这篇综述论文中，详细介绍了基于这些响应性 PDA 囊泡检测各种微生物（细菌，直接检测或通过释放的毒素或通过其 DNA 检测，以及病毒）和抗菌肽和抗病毒肽的原理。给出了囊泡悬浮或固定时获得的分析性能，并与主要基于囊泡包封法的响应性囊泡进行了比较。然后讨论了许多未来的挑战。
