Résibois A, Rypens F, Pochet R
Laboratoire d'Histologie, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Cell Tissue Res. 1988 Mar;251(3):611-20. doi: 10.1007/BF00214010.
It is well known that calbindin immunoreactivity is highly concentrated in the duodenal absorptive cells of young birds. We have shown that in the adult intestine of three avian species, calbindin content is much more variable. In addition to absorptive cells, we have detected throughout the gut of both sexes of the domestic fowl and in the large intestine of the Japanese quail a second type of calbindin-positive epithelial cell which has the shape of a typical endocrine cell. These cells were particularly abundant in the large intestine, in contrast to the usual distribution of endocrine cells along the gut. Calbindin was also detected in the nervous system of the intestine. Calbindin-positive nerve fibres were rare in the duodenum and ileum, numerous in plexuses and nerve processes in both muscular layers and lamina propria of the large intestine in domestic fowl and Japanese quail. In the mallard, nerve fibres were rarely calbindin positive while definitively positive for VIP. Calbindin of the peripheral nervous system of the domestic fowl and Japanese quail comigrates with the duodenal calbindin (27,000 dalton) in SDS gel electrophoresis.
众所周知，钙结合蛋白免疫反应性高度集中在幼鸟的十二指肠吸收细胞中。我们已经表明，在三种鸟类的成年肠道中，钙结合蛋白含量的变化要大得多。除了吸收细胞外，我们在家禽两性的整个肠道以及日本鹌鹑的大肠中都检测到了第二种类型的钙结合蛋白阳性上皮细胞，其形状为典型的内分泌细胞。与内分泌细胞沿肠道的通常分布相反，这些细胞在大肠中特别丰富。在肠道神经系统中也检测到了钙结合蛋白。钙结合蛋白阳性神经纤维在十二指肠和回肠中很少见，在家禽和日本鹌鹑的大肠肌层和固有层的神经丛和神经突起中大量存在。在绿头鸭中，神经纤维很少有钙结合蛋白阳性，而对血管活性肠肽（VIP）则肯定呈阳性。在家禽和日本鹌鹑的外周神经系统中，钙结合蛋白在SDS凝胶电泳中与十二指肠钙结合蛋白（27,000道尔顿）共迁移。
