Parmentier M, Ghysens M, Rypens F, Lawson D E, Pasteels J L, Pochet R
Gen Comp Endocrinol. 1987 Mar;65(3):399-407. doi: 10.1016/0016-6480(87)90125-0.
Calbindin immunoreactivity was investigated in various vertebrates. Positive labeling was observed in the absorptive cells of the duodenum of all birds and reptiles but not in mammals, amphibia, or fish. Staining was present in the kidney distal convoluted tubule from amphibia and higher vertebrates. Fish kidney was negative. In the central nervous system of all species investigated, cellular bodies and fibers were Calbindin positive. Their distribution was quite broad and correlates well with the previously reported mapping for chick and rat. Western blot analysis revealed two Calbindins in brain from mammals, birds, reptiles, and amphibia (27,000 and 29,000 Da). Only one band was detected in fish. We conclude that Calbindin from the evolutionary point of view is primarily a neuronal protein, with a highly conservative character.
研究了不同脊椎动物中钙结合蛋白的免疫反应性。在所有鸟类和爬行动物的十二指肠吸收细胞中观察到阳性标记，但在哺乳动物、两栖动物或鱼类中未观察到。两栖动物和高等脊椎动物的肾远曲小管中有染色。鱼类肾脏为阴性。在所研究的所有物种的中枢神经系统中，细胞体和纤维均为钙结合蛋白阳性。它们的分布相当广泛，与先前报道的鸡和大鼠的图谱相关性良好。蛋白质免疫印迹分析显示，哺乳动物、鸟类、爬行动物和两栖动物的大脑中有两种钙结合蛋白（27,000和29,000道尔顿）。在鱼类中仅检测到一条带。我们得出结论，从进化的角度来看，钙结合蛋白主要是一种神经元蛋白，具有高度保守的特性。
