Armas-Portela R, Paweletz N, Zimmermann H P, Ghosh S
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
Cell Motil Cytoskeleton. 1988;9(3):254-63. doi: 10.1002/cm.970090307.
The peroxidase-antiperoxidase (PAP) method for the detection of polymerized tubulin has been used to study the microtubule rearrangements during mitosis in PtK1 and HeLa multinucleate cells obtained by polyethyleneglycol (PEG)-mediated fusion. We demonstrate here that the transition of the microtubular cytoskeleton from interphase to mitosis is an inducible event and independent of the factor(s) responsible for chromatin condensation and nuclear envelope breakdown. However, for the induction of the microtubule rearrangements nuclear envelope breakdown is required. At midprophase, cytoskeletal microtubule rearrangements start for multinucleate PtK1 cells, whereas in HeLa cells such changes are delayed, and a more abrupt transition is observed here. After complete nuclear envelope breakdown (prometaphase) mitotic asters and spindles but no cytoplasmic (interphase) microtubuli can be observed in both systems. Metaphase is characterized by an interaction between the different mitotic poles which show the form of bipolar spindles, but individual separated mitotic poles far removed from the chromatin can also be seen.
过氧化物酶抗过氧化物酶（PAP）法用于检测聚合微管蛋白，已被用于研究聚乙二醇（PEG）介导融合获得的PtK1和HeLa多核细胞有丝分裂期间的微管重排。我们在此证明，微管细胞骨架从间期到有丝分裂的转变是一个可诱导事件，且独立于负责染色质凝聚和核膜破裂的因子。然而，对于微管重排的诱导，核膜破裂是必需的。在早中期，多核PtK1细胞的细胞骨架微管开始重排，而在HeLa细胞中这种变化延迟，且在此观察到更突然的转变。在核膜完全破裂（前中期）后，在两个系统中均可观察到有丝分裂星体和纺锤体，但无细胞质（间期）微管。中期的特征是不同有丝分裂极之间的相互作用，呈现双极纺锤体形式，但也可见远离染色质的单个分离有丝分裂极。
