Wang E E, Hammerberg O, Lyn P, Peng H Q, Hunter D, Richardson H
Division of Infectious Diseases, Hospital for Sick Children, Toronto, Ontario.
Clin Invest Med. 1988 Feb;11(1):52-6.
Group B streptococcal disease is the most frequent infectious cause of morbidity and mortality of newborns in North America. There is evidence that vertical transmission, the major route of neonatal acquisition, may be interrupted by administration of antibiotics during the intrapartum period. A modified starch serum medium has been developed that will allow the rapid identification of group B streptococci, by observation of a color change, in vaginal and rectal swabs. This medium was compared to a "gold standard" of routine culture. The sensitivity and specificity of the test were found to be 100% and 99%, respectively, in 237 specimens from women attending different clinics who submitted vaginal swabs. All color changes occurred within 6 hours. Vaginal and rectal carriage of the organism were found in 8% and 12%, respectively, of women in preterm labor. In this population the sensitivity and specificity of the test for vaginal specimens were 97% and 98%, respectively. The corresponding values for rectal swabs were 94% and 100%. The test can potentially select carrier mothers who could benefit the most from intrapartum antibiotics. This medium may also be used for transport to enhance detection of colonization.
B族链球菌病是北美新生儿发病和死亡最常见的感染原因。有证据表明，垂直传播作为新生儿感染的主要途径，可通过产时使用抗生素来阻断。已开发出一种改良淀粉血清培养基，通过观察阴道和直肠拭子颜色变化，可快速鉴定B族链球菌。将该培养基与常规培养的“金标准”进行比较。在来自不同诊所的237例提交阴道拭子的女性标本中，该检测的敏感性和特异性分别为100%和99%。所有颜色变化均在6小时内出现。早产女性中，分别有8%和12%的人阴道和直肠携带该菌。在该人群中，阴道标本检测的敏感性和特异性分别为97%和98%。直肠拭子的相应值为94%和100%。该检测有可能筛选出最能从产时抗生素中获益的携带菌母亲。这种培养基也可用于转运，以提高定植检测率。
