Andreu Domingo A, Saleedo Abizanda S, Heredia Prim F, González Morlans J, Bartolomé Comas R M, Cabero Roura L
Servicio de Microbiología, Hospitals Vall d'Hebron, Barcelona.
An Esp Pediatr. 1997 Apr;46(4):378-82.
The goal of this study was to evaluate three methods for rapid group B streptococcus (GBS) intrapartum vaginal detection.
In 330 women, at risk of delivering an infant with GBS disease, vaginal exudates were collected and a culture performed. The following rapid tests were also performed: 1) Equate Strep B immunoassay in 133 samples. 2) Icon Strep B immunoassay in 192 samples. 3) Co-agglutination with Phadebact Strep B, with a previous incubation (> 4 hours) of the vaginal swabs in Lim Group B Strep broth, in 88 samples. In some patients, two of these methods were performed simultaneously.
GBS was detected in 37 women (11.2%) by culture. The sensitivity of Equate Strep B was 47%, Icon Strep B was 35% and co-agglutination with Phadebact Strep B was 38%. The specificity was 91%, 99% and 100% for each one of these methods. PPV 44%, 90% and 100%, respectively and NPV 92%, 91% and 90%, respectively.
In conclusion, none of these methods was shown sensitive enough to be used for the routine detection of GBS. Therefore, in order to know the GBS carrier status and prevent its vertical transmission, the practice of vaginal culture during late pregnancy is mandatory.
本研究的目的是评估三种快速检测分娩期B族链球菌（GBS）阴道感染的方法。
选取330名有分娩GBS感染婴儿风险的女性，收集阴道分泌物并进行培养。还进行了以下快速检测：1）对133份样本进行Equate B族链球菌免疫测定。2）对192份样本进行Icon B族链球菌免疫测定。3）对88份样本进行与Phadebact B族链球菌的协同凝集试验，之前将阴道拭子在Lim B族链球菌肉汤中孵育（>4小时）。部分患者同时进行了其中两种方法的检测。
通过培养在37名女性（11.2%）中检测到GBS。Equate B族链球菌的敏感性为47%，Icon B族链球菌为35%，与Phadebact B族链球菌的协同凝集试验为38%。这些方法的特异性分别为91%、99%和100%。阳性预测值分别为44%、90%和100%，阴性预测值分别为92%、91%和90%。
总之，这些方法均未显示出足够的敏感性用于GBS的常规检测。因此，为了解GBS携带状态并预防其垂直传播，妊娠晚期进行阴道培养是必要的。
