de la Rosa M, Perez M, Carazo C, Pareja L, Peis J I, Hernandez F
Microbiology Service, Virgen de las Nieves Hospital, Granada, Spain.
J Clin Microbiol. 1992 Apr;30(4):1019-21. doi: 10.1128/jcm.30.4.1019-1021.1992.
A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.
本文描述了一种含甲氨蝶呤的培养基,用于基于色素检测从临床标本中检测B族β溶血性链球菌。该培养基含有蛋白胨、淀粉、血清、硫酸镁、葡萄糖、丙酮酸、甲氨蝶呤(作为色素增强剂)、磷酸-吗啉-丙磺酸缓冲液和选择性试剂。B族β溶血性链球菌的回收率与使用选择性肉汤获得的回收率相当。