Department of Biology, University of Konstanz, 78464 Konstanz, Germany.
Department of Microorganisms, Leibniz Institute DSMZ-German Culture Collection for Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Syst Appl Microbiol. 2020 Sep;43(5):126105. doi: 10.1016/j.syapm.2020.126105. Epub 2020 Jun 21.
Two strains of sulfate-reducing bacteria (J.5.4.2-L4.2.8 and J.3.6.1-H7) were isolated from a pyrite-forming enrichment culture and were compared phylogenetically and physiologically to the closest related type strain Desulfovibrio sulfodismutans DSM 3696. The isolated strains were vibrio-shaped, motile rods that stained Gram-negative. Growth occurred from 15 to 37°C and within a pH range of 6.5-8.5. Both strains used sulfate, thiosulfate, sulfite, and dimethyl sulfoxide (DMSO) as electron acceptor when grown with lactate. Lactate was incompletely oxidized to acetate. Formate and H were used as electron donor in the presence of acetate. Dismutation of thiosulfate and pyrosulfite was observed. The two new isolates differed from D. sulfodismutans by the utilization of DMSO as electron acceptor, 82% genome-wide average nucleotide identity (ANI) and 32% digital DNA-DNA hybridization (dDDH), thus representing a novel species. The type strain of the type species Desulfovibrio desulfuricans Essex6 revealed merely 88% 16S rRNA gene identity and 49% genome-wide average amino acid identity (AAI) to the new isolates as well as to D. sulfodismutans. Furthermore, the dominance of menaquinone MK-7 over MK-6 and the dominance of ai-C fatty acids were observed not only in the two new isolated strains but also in D. sulfodismutans. Therefore, the definition of a new genus is indicated for which the name Desulfolutivibrio is proposed. We propose for strains J.5.4.2-L4.2.8 and J.3.6.1-H7 the name Desulfolutivibrio sulfoxidireducens gen. nov. sp. nov. with strain J.5.4.2-L4.2.8 defined as type strain. In addition, we propose the reclassification of Desulfovibrio sulfodismutans as Desulfolutivibrio sulfodismutans comb. nov.
从一个形成黄铁矿的富集培养物中分离出两株硫酸盐还原菌（J.5.4.2-L4.2.8 和 J.3.6.1-H7），并从系统发育和生理学角度与最接近的相关模式菌株脱硫弧菌 DSM 3696 进行了比较。分离出的菌株为弧菌形、运动的杆状，革兰氏阴性染色。生长发生在 15 到 37°C 之间，pH 值范围为 6.5-8.5。当用乳酸盐生长时，两株菌都可以使用硫酸盐、硫代硫酸盐、亚硫酸盐和二甲基亚砜 (DMSO) 作为电子受体。乳酸盐不完全氧化为乙酸盐。在存在乙酸盐的情况下，使用甲酸盐和 H 作为电子供体。观察到硫代硫酸盐和焦硫酸盐的歧化作用。这两个新的分离株与脱硫弧菌的不同之处在于 DMSO 作为电子受体的利用、82%的全基因组平均核苷酸同一性 (ANI) 和 32%的数字 DNA-DNA 杂交 (dDDH)，因此代表一个新的种。脱硫弧菌 Desulfovibrio desulfuricans Essex6 的模式株与新分离株以及脱硫弧菌的 16S rRNA 基因同一性仅为 88%，全基因组平均氨基酸同一性 (AAI) 为 49%。此外，不仅在两个新的分离株中，而且在脱硫弧菌中观察到menaquinone MK-7 对 MK-6 的优势和 ai-C 脂肪酸的优势。因此，表明需要为一个新属定义名称，建议使用 Desulfolutivibrio。我们建议将 J.5.4.2-L4.2.8 和 J.3.6.1-H7 菌株命名为 Desulfolutivibrio sulfoxidireducens gen. nov. sp. nov.，将 J.5.4.2-L4.2.8 菌株定义为模式菌株。此外，我们建议将脱硫弧菌 Desulfovibrio sulfodismutans 重新分类为 Desulfolutivibrio sulfodismutans comb. nov.
