Weisberger J, Wu C D, Liu Z, Wong J Y, Melamed M R, Darzynkiewicz Z, Gorczyca W
Division of Hematopathology, IMPATH Inc., New York, NY, USA.
Int J Oncol. 2000 Dec;17(6):1165-77. doi: 10.3892/ijo.17.6.1165.
The introduction of monoclonal antibodies (mAbs) for cell immunophenotyping and use of flow cytometry with the progressively improving software for multivariate analyses have revolutionized the diagnosis and influenced the classification of hematologic neoplasms. In this review we focus on the practical application of flow cytometry in the diagnosis and classification of malignant lymphomas and related lymphoproliferative disorders with special emphasis on differential diagnosis. A general approach to the utilization of flow cytometry (FC) in hematopathology with an algorithm to diagnose the most common neoplasms is presented. We discuss precursor B-cell neoplasms, mature B-cell neoplasms (SLL/CLL, mantle cell lymphoma, marginal zone lymphoma, hairy cell leukemia, diffuse large B-cell lymphoma, plasma cell dyscrasias and lymphomas with plasmacytic differentiation), precursor T-lymphoblastic leukemia and mature (peripheral) T-cell neoplasms, including T-SLL/PLL, anaplastic cell lymphomas and large granular cell leukemia/lymphoma. The text is accompanied by characteristic FC scatterplots of the discussed entities.
单克隆抗体（mAb）用于细胞免疫表型分析，以及流式细胞术与不断改进的多变量分析软件的结合，彻底改变了血液系统肿瘤的诊断，并影响了其分类。在本综述中，我们重点关注流式细胞术在恶性淋巴瘤及相关淋巴增殖性疾病诊断和分类中的实际应用，特别强调鉴别诊断。本文介绍了在血液病理学中利用流式细胞术（FC）诊断最常见肿瘤的一般方法及算法。我们讨论了前体B细胞肿瘤、成熟B细胞肿瘤（小淋巴细胞淋巴瘤/慢性淋巴细胞白血病、套细胞淋巴瘤、边缘区淋巴瘤、毛细胞白血病、弥漫性大B细胞淋巴瘤、浆细胞异常增生症以及具有浆细胞分化的淋巴瘤）、前体T淋巴细胞白血病和成熟（外周）T细胞肿瘤，包括T-小淋巴细胞淋巴瘤/幼淋巴细胞白血病、间变性大细胞淋巴瘤和大颗粒细胞白血病/淋巴瘤。文中还配有所讨论实体的特征性FC散点图。
